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@MASTERSTHESIS{Kellhammer:572929,
author = {Kellhammer, Diana},
othercontributors = {Henri, Müller},
title = {{B}ildverarbeitung filamentöser {S}chimmelpilze kultiviert
mit {M}ikro- und {M}akropartikeln},
school = {Technische Universität München},
type = {Masterarbeit},
address = {Freising},
publisher = {School of Life Sciences},
reportid = {PUBDB-2023-00816},
pages = {87},
year = {2022},
note = {konnte die Arbeit leider nicht online finden.;
Masterarbeit, Technische Universität München, 2022},
abstract = {The numerous advantages of filamentous fungal-pellets for
the production of various products, such as citric acid,
attract the attention of researchers and industrial
engineers. In this regard, it is important to study the
morphology of the fungal pellets, as it has a great impact
on productivity. This can best be analyzed using
three-dimensional images taken with synchrotron
radiation-based X-ray microtomography. The morphology of the
fungal pellets can also be greatly influenced by the
addition of micro- and macro particles. Therefore, in this
thesis, the morphology of Aspergillus niger fungal-pellets
from different series of experiments was investigated using
three-dimensional images acquired at the synchrotron
(Deutsches Elektron Synchrotron DESY, Hamburg, Germany). For
morphology analysis, a method was developed in MATLAB® to
segment the embedded glass. Subsequently, the hyphal, hyphal
tip, and branching densities, as well as the distribution of
the embedded glass over the radius, were determined. In the
first series of experiment fungal cells were cultivated with
and without microparticles for different cultivation times.
The result showed that pellets grow faster at the beginning
of cultivation and also continue to grow not only at the
edge of the pellet but also inside the pellet. Furthermore,
pellets with different concentrations of microparticles were
cultivated. This resulted in smaller, less dense pellets for
higher concentrations. In addition, pellets were cultivated
with surface-modified microparticles. This cultivation
resulted in pellets with low hyphae density in the center
and relatively high hyphae density at the edge of the
pellet. In addition, ring-shaped inclusions of the glass
resulted for the cultivation of pellets for some
modifications. Finally, pellets with macroparticles of
different sizes were cultivated. This resulted in less
dense, large pellets for relatively small macroparticles and
dense, small pellets for relatively large macroparticles.
Finally, a new method was developed to divide the fungal
pellets into smaller volume parts in order to investigate
different properties in more detail.},
cin = {DOOR ; HAS-User},
cid = {I:(DE-H253)HAS-User-20120731},
pnm = {6G3 - PETRA III (DESY) (POF4-6G3) / FS-Proposal: I-20211150
(I-20211150) / FS-Proposal: I-20200214 (I-20200214)},
pid = {G:(DE-HGF)POF4-6G3 / G:(DE-H253)I-20211150 /
G:(DE-H253)I-20200214},
experiment = {EXP:(DE-H253)P-P05-20150101},
typ = {PUB:(DE-HGF)19},
url = {https://bib-pubdb1.desy.de/record/572929},
}