TY  - THES
AU  - Kellhammer, Diana
TI  - Bildverarbeitung filamentöser Schimmelpilze kultiviert mit Mikro- und Makropartikeln
PB  - Technische Universität München
VL  - Masterarbeit
CY  - Freising
M1  - PUBDB-2023-00816
SP  - 87
PY  - 2022
N1  - konnte die Arbeit leider nicht online finden.
N1  - Masterarbeit, Technische Universität München, 2022
AB  - The numerous advantages of filamentous fungal-pellets for the production of various products, such as citric acid, attract the attention of researchers and industrial engineers. In this regard, it is important to study the morphology of the fungal pellets, as it has a great impact on productivity. This can best be analyzed using three-dimensional images taken with synchrotron radiation-based X-ray microtomography. The morphology of the fungal pellets can also be greatly influenced by the addition of micro- and macro particles. Therefore, in this thesis, the morphology of Aspergillus niger fungal-pellets from different series of experiments was investigated using three-dimensional images acquired at the synchrotron (Deutsches Elektron Synchrotron DESY, Hamburg, Germany). For morphology analysis, a method was developed in MATLAB® to segment the embedded glass. Subsequently, the hyphal, hyphal tip, and branching densities, as well as the distribution of the embedded glass over the radius, were determined. In the first series of experiment fungal cells were cultivated with and without microparticles for different cultivation times. The result showed that pellets grow faster at the beginning of cultivation and also continue to grow not only at the edge of the pellet but also inside the pellet. Furthermore, pellets with different concentrations of microparticles were cultivated. This resulted in smaller, less dense pellets for higher concentrations. In addition, pellets were cultivated with surface-modified microparticles. This cultivation resulted in pellets with low hyphae density in the center and relatively high hyphae density at the edge of the pellet. In addition, ring-shaped inclusions of the glass resulted for the cultivation of pellets for some modifications. Finally, pellets with macroparticles of different sizes were cultivated. This resulted in less dense, large pellets for relatively small macroparticles and dense, small pellets for relatively large macroparticles. Finally, a new method was developed to divide the fungal pellets into smaller volume parts in order to investigate different properties in more detail.
LB  - PUB:(DE-HGF)19
UR  - https://bib-pubdb1.desy.de/record/572929
ER  -