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@INPROCEEDINGS{Taberman:517549,
author = {Taberman, Helena and Borges Perdomo, Clemente and
Chatziefthymiou, Spyridon and Crosas Ubeda, Eva and
Gruzinov, Andrey and Kistner, Bernhard and Pompidor,
Guillaume and Song, jialing and Hakanpaeae, Johanna},
title = {{T}he {H}igh-{T}roughput {M}acromolecular {C}rystallography
{B}eamline {P}11 at {PETRA} {III}},
reportid = {PUBDB-2023-00532},
year = {2022},
abstract = {P11 at PETRA III (DESY, Hamburg) is a high-throughput
instrument for macromolecular crystallography (1). P11 has
tuneable photon energy between 5.5 - 28 keV having the
Eiger2 X 16M as the stationary detector and the possibility
of using a CdTe-detector for higher energies. Beam size from
200 x 200 μm to 4 x 9 μm can be used with a maximum photon
flux of 1.3 x $10^13$ ph/s at 12 keV energy. The automatic
sample changer at P11 is based on the unipuck format with a
total capacity of 23 pucks (368 samples) having a
mount-unmount cycle of approximately 36 s, which brings the
beamtime spent per sample down to ca. 2min. Furthermore, in
close proximity of the beamline, P11 has a user laboratory
with e.g. a crystal shifter for semi-automated crystal
harvesting.During the past two years mode of operation at
P11 has changed from fully on-site to almost exclusively
remote. Remote connection using FastX-access via a dedicated
remote machine was established in 2020, which has enabled
near normal user operation throughout the pandemic. Users
are supported by a virtual meeting and the scientific
accounts can be used during and after the beamtime for
manual data processing on Maxwell, the central computational
cluster, where the autoprocessing is also migrated to the
dedicated P11 nodes.P11 is a very diverse environment and
allows the adjustment to various non-standard experiments
e.g. via the long term proposal (LTP) scheme. For example,
serial synchrotron crystallography at P11 is enabled with
sample delivery through various types of solid supports or
the tape-drive setup, which enables time-resolved
experiments by the mix-and diffuse method (2), and has been
developed through the LTP scheme along with the real-time
autoprocessing of serial data with CrystFEL (3). This year
MXCuBE will be employed as the default control software with
the integration to ISPyB for tracking shipments,
communicating the sample details to MXCuBE, as well as
acting as a data archive. The establishment of parallel
autoprocessing pipelines in addition to the currently used,
XDSAPP-based (4) pipeline, and the implementation of
strategy calculation including dose estimation are also
planned. Furthermore, these software developments are
synchronising P11 with the EMBL PETRA III beamlines for the
future foundation of a uniform structural biology village at
PETRA IV.[1] Burkhardt A., et al., Status of the
crystallography beamlines at PETRA III. Eur. Phys. J. Plus
131, 56 (2016)[2] Beyerlein K. R., et al., Mix-and-diffuse
serial synchrotron crystallography. IUCrJ 4, 769-777
(2017)[3] White T. A., et al., Recent developments in
CrystFEL. J. Appl. Cryst. 49, 680-689 (2016)[4] Sparta KM,
et al., XDSAPP2.0. J. Appl. Cryst. 49, 1085-1092 (2016)},
month = {Jul},
date = {2022-07-23},
organization = {33rd European Crystallographic
Meeting, Versailles (France), 23 Jul
2022 - 27 Jul 2022},
cin = {FS-PETRA-D / ITT},
cid = {I:(DE-H253)FS-PETRA-D-20210408 / I:(DE-H253)ITT-20160816},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633) / 6G3 - PETRA III (DESY) (POF4-6G3)},
pid = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF4-6G3},
experiment = {EXP:(DE-H253)P-P11-20150101},
typ = {PUB:(DE-HGF)6},
url = {https://bib-pubdb1.desy.de/record/517549},
}