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@ARTICLE{Ravichandran:491473,
      author       = {Ravichandran, Mirunalini and Rafalski, Dominik and Davies,
                      Claudia I. and Ortega-Recalde, Oscar and Nan, Xinsheng and
                      Glanfield, Cassandra R. and Kotter, Annika and Misztal,
                      Katarzyna and Wang, Andrew H. and Wojciechowski, Marek and
                      Rażew, Michał and Mayyas, Issam M. and Kardailsky, Olga
                      and Schwartz, Uwe and Zembrzycki, Krzysztof and Morison, Ian
                      M. and Helm, Mark and Weichenhan, Dieter and Jurkowska,
                      Renata Z. and Krueger, Felix and Plass, Christoph and
                      Zacharias, Martin and Bochtler, Matthias and Hore, Timothy
                      A. and Jurkowski, Tomasz P.},
      title        = {{P}ronounced sequence specificity of the {TET} enzyme
                      catalytic domain guides its cellular function},
      journal      = {Science advances},
      volume       = {8},
      number       = {36},
      issn         = {2375-2548},
      address      = {Washington, DC [u.a.]},
      publisher    = {Assoc.},
      reportid     = {PUBDB-2023-00182},
      pages        = {eabm2427},
      year         = {2022},
      abstract     = {TET (ten-eleven translocation) enzymes catalyze the
                      oxidation of 5-methylcytosine bases in DNA, thus driving
                      active and passive DNA demethylation. Here, we report that
                      the catalytic domain of mammalian TET enzymes favor CGs
                      embedded within basic helix-loop-helix and basic leucine
                      zipper domain transcription factor–binding sites, with up
                      to 250-fold preference in vitro. Crystal structures and
                      molecular dynamics calculations show that sequence
                      preference is caused by intrasubstrate interactions and CG
                      flanking sequence indirectly affecting enzyme conformation.
                      TET sequence preferences are physiologically relevant as
                      they explain the rates of DNA demethylation in TET-rescue
                      experiments in culture and in vivo within the zygote and
                      germ line. Most and least favorable TET motifs represent DNA
                      sites that are bound by methylation-sensitive
                      immediate-early transcription factors and octamer-binding
                      transcription factor 4 (OCT4), respectively, illuminating
                      TET function in transcriptional responses and pluripotency
                      support.},
      cin          = {DOOR ; HAS-User},
      ddc          = {500},
      cid          = {I:(DE-H253)HAS-User-20120731},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3)},
      pid          = {G:(DE-HGF)POF4-6G3},
      experiment   = {EXP:(DE-H253)P-P11-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36070377},
      UT           = {WOS:000911968500003},
      doi          = {10.1126/sciadv.abm2427},
      url          = {https://bib-pubdb1.desy.de/record/491473},
}