Preprint

PUBDB-2022-07234

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Gelation dynamics upon pressure-induced liquid-liquid phase separation in a water-lysozyme solution

Moron, M. (Corresponding author)Extern* ; Al-Masoodi, A.Extern*XFEL.EU* ; Lovato, C.Extern*XFEL.EU* ; Reiser, M.XFEL.EU* ; Randolph, L.Extern*XFEL.EU*DESY* ; Surmeier, G.Extern* ; Bolle, J.Extern* ; Westermeier, F.XFEL.EU*DESY* ; Sprung, M.XFEL.EU*DESY* ; Winter, R.Extern* ; Paulus, M.Extern* ; Gutt, C.Extern*XFEL.EU*

2022

This record in other databases:

Report No.: arXiv:2107.05026

Abstract: Employing X-ray photon correlation spectroscopy we measure the kinetics and dynamics of a pressure-induced liquid-liquid phase separation (LLPS) in a water-lysozyme solution. Scattering invariants and kinetic information provide evidence that the system reaches the phase boundary upon pressure-induced LLPS with no sign of arrest. The coarsening slows down with increasing quench depths. The $g_2$-functions display a two-step decay with a gradually increasing non-ergodicity parameter typical for gelation. We observe fast superdiffusive ($\gamma \geq 3/2$) and slow subdiffusive ($\gamma < 0.6$) motion associated with fast viscoelastic fluctuations of the network and a slow viscous coarsening process, respectively. The dynamics age linear with time $\tau \propto t_\mathrm{w}$ and we observe the onset of viscoelastic relaxation for deeper quenches. Our results suggest that the protein solution gels upon reaching the phase boundary.

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Contributing Institute(s):

1. DOOR-User (DOOR ; HAS-User)
2. PETRA-S (FS-PETRA-S)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. 6G13 - Accelerator of European XFEL (POF4-6G13) (POF4-6G13)

Experiment(s):

1. PETRA Beamline P10 (PETRA III)

Appears in the scientific report 2022

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Database coverage:
Published

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Moron, M. (Corresponding author)Extern* ; Al-Masoodi, A.Extern*XFEL.EU* ; Lovato, C.Extern*XFEL.EU* ; Reiser, M.XFEL.EU* ; Randolph, L.Extern*XFEL.EU*DESY* ; Surmeier, G.Extern* ; Bolle, J.Extern* ; Westermeier, F.XFEL.EU*DESY* ; Sprung, M.XFEL.EU*DESY* ; Winter, R.Extern* ; Paulus, M.Extern* ; Gutt, C. (Corresponding author)Extern*XFEL.EU*
Gelation Dynamics upon Pressure-Induced Liquid–Liquid Phase Separation in a Water–Lysozyme Solution
The journal of physical chemistry <Washington, DC> / B 126(22), 4160 - 4167 (2022) [10.1021/acs.jpcb.2c01947]2022     Files  Fulltext by arXiv.org BibTeX | EndNote: XML, Text | RIS

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