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@ARTICLE{Seyfert:486214,
      author       = {Seyfert, Carsten E and Porten, Christoph and Yuan, Biao and
                      Deckarm, Selina and Panter, Fabian and Bader, Chantal and
                      Coetzee, Janetta and Deschner, Felix and Tehrani, Kamaleddin
                      and Higgins, Paul G and Seifert, Harald and Marlovits,
                      Thomas and Herrmann, Jennifer and Müller, Rolf},
      title        = {{D}arobactins {E}xhibiting {S}uperior {A}ntibiotic
                      {A}ctivity by {C}ryo‐{EM} {S}tructure {G}uided
                      {B}iosynthetic {E}ngineering},
      journal      = {Angewandte Chemie / International edition},
      volume       = {62},
      number       = {2},
      issn         = {1433-7851},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {PUBDB-2022-07156},
      pages        = {e202214094},
      year         = {2023},
      abstract     = {Over recent decades, the pipeline of antibiotics acting
                      against Gram-negative bacteria is running dry, as most
                      discovered candidate antibiotics suffer from insufficient
                      potency, pharmacokinetic properties, or toxicity. The
                      darobactins, a promising new small peptide class of drug
                      candidates, bind to novel antibiotic target BamA, an outer
                      membrane protein. Previously, we reported that biosynthetic
                      engineering in a heterologous host generated novel
                      darobactins with enhanced antibacterial activity. Here we
                      utilize an optimized purification method and present cryo-EM
                      structures of the Bam complex with darobactin 9 (D9), which
                      served as a blueprint for the biotechnological generation of
                      twenty new darobactins including halogenated analogs. The
                      newly engineered darobactin 22 binds more tightly to BamA
                      and outperforms the favorable activity profile of D9 against
                      clinically relevant pathogens such as carbapenem-resistant
                      Acinetobacter baumannii up to 32-fold, without observing
                      toxic effects.},
      cin          = {FS-CS / CSSB-UKE-TM},
      ddc          = {540},
      cid          = {I:(DE-H253)FS-CS-20210408 /
                      I:(DE-H253)CSSB-UKE-TM-20210520},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633)},
      pid          = {G:(DE-HGF)POF4-633},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36308277},
      UT           = {WOS:000893447000001},
      doi          = {10.1002/anie.202214094},
      url          = {https://bib-pubdb1.desy.de/record/486214},
}