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000486058 1001_ $$aFruergaard, Marlene U.$$b0
000486058 245__ $$aThe Na$^+$,K$^+$-ATPase in complex with beryllium fluoride mimics an ATPase phosphorylated state 
000486058 260__ $$aBethesda, MD.$$bAmerican Soc. for Biochemistry and Molecular Biology$$c2022
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000486058 520__ $$aThe Na$^+$,K$^+$-ATPase generates electrochemical gradients of Na$^+$ and K$^+$ across the plasma membrane via a functional cycle that includes various phosphoenzyme intermediates. However, the structure and function of these intermediates and how metal fluorides mimick them require further investigation. Here, we describe a 4.0 Å resolution crystal structure and functional properties of the pig kidney Na$^+$,K$^+$-ATPase stabilized by the inhibitor beryllium fluoride (denoted E2–BeF$_x$). E2–BeF$_x$ is expected to mimic properties of the E2P phosphoenzyme, yet with unknown characteristics of ion and ligand binding. The structure resembles the E2P form obtained by phosphorylation from inorganic phosphate (P$_i$) and stabilized by cardiotonic steroids, including a low-affinity Mg$^{2+}$ site near ion binding site II. Our anomalous Fourier analysis of the crystals soaked in Rb$^+$ (a K$^+$ congener) followed by a low-resolution rigid-body refinement (6.9–7.5 Å) revealed preocclusion transitions leading to activation of the dephosphorylation reaction. We show that the Mg$^{2+}$ location indicates a site of initial K$^+$ recognition and acceptance upon binding to the outward-open E2P state after Na$^+$ release. Furthermore, using binding and activity studies, we find that the BeF$_x$-inhibited enzyme is also able to bind ADP/ATP and Na$^+$. These results relate the E2–BeF$_x$ complex to a transient K$^+$- and ADP-sensitive E∗P intermediate of the functional cycle of the Na$^+$,K$^+$-ATPase, prior to E2P.
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000486058 7001_ $$aDach, Ingrid$$b1
000486058 7001_ $$aAndersen, Jacob L.$$b2
000486058 7001_ $$aOzol, Mette$$b3
000486058 7001_ $$aShahsavar, Azadeh$$b4
000486058 7001_ $$aQuistgaard, Esben M.$$b5
000486058 7001_ $$aPoulsen, Hanne$$b6
000486058 7001_ $$00000-0002-1706-6108$$aFedosova, Natalya U.$$b7$$eCorresponding author
000486058 7001_ $$0P:(DE-H253)PIP1087253$$aNissen, Poul$$b8$$eCorresponding author
000486058 77318 $$2Crossref$$3journal-article$$a10.1016/j.jbc.2022.102317$$bElsevier BV$$d2022-09-01$$n9$$p102317$$tJournal of Biological Chemistry$$v298$$x0021-9258$$y2022
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