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@ARTICLE{Petzoldt:480707,
author = {Petzoldt, Astrid G. and Götz, Torsten W. B. and Driller,
Jan Heiner and Lützkendorf, Janine and Reddy-Alla, Suneel
and Matkovic-Rachid, Tanja and Liu, Sunbin and Knoche, Elena
and Mertel, Sara and Ugorets, Vladimir and Lehmann, Martin
and Ramesh, Niraja and Beuschel, Christine Brigitte and
Kuropka, Benno and Freund, Christian and Stelzl, Ulrich and
Loll, Bernhard and Liu, Fan and Wahl, Markus C. and Sigrist,
Stephan J.},
title = {{RIM}-binding protein couples synaptic vesicle recruitment
to release sites},
journal = {The journal of cell biology},
volume = {219},
number = {7},
issn = {0021-9525},
address = {New York, NY},
publisher = {Rockefeller Univ. Press},
reportid = {PUBDB-2022-03945},
pages = {e201902059},
year = {2020},
abstract = {At presynaptic active zones, arrays of large conserved
scaffold proteins mediate fast and temporally precise
release of synaptic vesicles (SVs). SV release sites could
be identified by clusters of Munc13, which allow SVs to dock
in defined nanoscale relation to Ca2+ channels. We here show
in Drosophila that RIM-binding protein (RIM-BP) connects
release sites physically and functionally to the ELKS family
Bruchpilot (BRP)-based scaffold engaged in SV recruitment.
The RIM-BP N-terminal domain, while dispensable for SV
release site organization, was crucial for proper nanoscale
patterning of the BRP scaffold and needed for SV recruitment
of SVs under strong stimulation. Structural analysis further
showed that the RIM-BP fibronectin domains form a
“hinge” in the protein center, while the C-terminal SH3
domain tandem binds RIM, Munc13, and Ca2+ channels release
machinery collectively. RIM-BPs’ conserved domain
architecture seemingly provides a relay to guide SVs from
membrane far scaffolds into membrane close release sites.},
cin = {EMBL-User},
ddc = {570},
cid = {I:(DE-H253)EMBL-User-20120814},
pnm = {6G3 - PETRA III (DESY) (POF4-6G3)},
pid = {G:(DE-HGF)POF4-6G3},
experiment = {EXP:(DE-H253)P-P14-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:32369542},
UT = {WOS:000548271800010},
doi = {10.1083/jcb.201902059},
url = {https://bib-pubdb1.desy.de/record/480707},
}