000474421 001__ 474421 000474421 005__ 20250724151343.0 000474421 0247_ $$2doi$$a10.1042/BST20210526 000474421 0247_ $$2ISSN$$a0300-5127 000474421 0247_ $$2ISSN$$a1470-8752 000474421 0247_ $$2datacite_doi$$a10.3204/PUBDB-2022-00779 000474421 0247_ $$2altmetric$$aaltmetric:119703343 000474421 0247_ $$2pmid$$apmid:34940787 000474421 0247_ $$2WOS$$aWOS:000736185200001 000474421 0247_ $$2openalex$$aopenalex:W4200599813 000474421 037__ $$aPUBDB-2022-00779 000474421 041__ $$aEnglish 000474421 082__ $$a540 000474421 1001_ $$aPeters, Thomas$$b0 000474421 245__ $$aNorovirus–glycan interactions — how strong are they really? 000474421 260__ $$aLondon$$bBiochemical Society$$c2022 000474421 3367_ $$2DRIVER$$aarticle 000474421 3367_ $$2DataCite$$aOutput Types/Journal article 000474421 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1675167549_18267 000474421 3367_ $$2BibTeX$$aARTICLE 000474421 3367_ $$2ORCID$$aJOURNAL_ARTICLE 000474421 3367_ $$00$$2EndNote$$aJournal Article 000474421 520__ $$aInfection with human noroviruses requires attachment to histo blood group antigens (HBGAs) via the major capsid protein VP1 as a primary step. Several crystal structures of VP1 protruding domain dimers, so called P-dimers, complexed with different HBGAs have been solved to atomic resolution. Corresponding binding affinities have been determined for HBGAs and other glycans exploiting different biophysical techniques, with mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy being most widely used. However, reported binding affinities are inconsistent. At the extreme, for the same system MS detects binding whereas NMR spectroscopy does not, suggesting a fundamental source of error. In this short essay, we will explain the reason for the observed differences and compile reliable and reproducible binding affinities. We will then highlight how a combination of MS techniques and NMR experiments affords unique insights into the process of HBGA binding by norovirus capsid proteins. 000474421 536__ $$0G:(DE-HGF)POF4-633$$a633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633)$$cPOF4-633$$fPOF IV$$x0 000474421 588__ $$aDataset connected to CrossRef, Journals: bib-pubdb1.desy.de 000474421 693__ $$0EXP:(DE-MLZ)NOSPEC-20140101$$5EXP:(DE-MLZ)NOSPEC-20140101$$eNo specific instrument$$x0 000474421 7001_ $$aCreutznacher, Robert$$b1 000474421 7001_ $$aMaass, Thorben$$b2 000474421 7001_ $$aMallagaray, Alvaro$$b3 000474421 7001_ $$aOgrissek, Patrick$$b4 000474421 7001_ $$0P:(DE-H253)PIP1031058$$aTaube, Stefan$$b5 000474421 7001_ $$0P:(DE-H253)PIP1097669$$aThiede, Lars$$b6 000474421 7001_ $$0P:(DE-H253)PIP1014042$$aUetrecht, Charlotte$$b7$$eCorresponding author 000474421 773__ $$0PERI:(DE-600)2007367-7$$a10.1042/BST20210526$$gp. 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