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@ARTICLE{Jenkins:473889,
      author       = {Jenkins, Joshua and Ishak, Mohd I. and Eales, Marcus and
                      Gholinia, Ali and Kulkarni, Satishkumar and Keller, Thomas
                      F. and May, Paul W. and Nobbs, Angela H. and Su, Bo},
      title        = {{R}esolving physical interactions between bacteria and
                      nanotopographies with focused ion beam scanning electron
                      microscopy},
      journal      = {iScience},
      volume       = {24},
      number       = {7},
      issn         = {2589-0042},
      address      = {St. Louis},
      publisher    = {Elsevier},
      reportid     = {PUBDB-2022-00331},
      pages        = {102818},
      year         = {2021},
      abstract     = {To robustly assess the antibacterial mechanisms of
                      nanotopographies, it is critical to analyze the
                      bacteria-nanotopography adhesion interface. Here, we utilize
                      focused ion beam milling combined with scanning electron
                      microscopy to generate three-dimensional reconstructions of
                      Staphylococcus aureus or Escherichia coli interacting with
                      nanotopographies. For the first time, 3D morphometric
                      analysis has been exploited to quantify the intrinsic
                      contact area between each nanostructure and the bacterial
                      envelope, providing an objective framework from which to
                      derive the possible antibacterial mechanisms of synthetic
                      nanotopographies. Surfaces with nanostructure densities
                      between 36 and 58 per μm2 and tip diameters between 27 and
                      50 nm mediated envelope deformation and penetration, while
                      surfaces with higher nanostructure densities (137 per μm2)
                      induced envelope penetration and mechanical rupture, leading
                      to marked reductions in cell volume due to cytosolic
                      leakage. On nanotopographies with densities of 8 per μm2
                      and tip diameters greater than 100 nm, bacteria
                      predominantly adhered between nanostructures, resulting in
                      cell impedance.},
      cin          = {DOOR ; HAS-User / FS-NL},
      ddc          = {050},
      cid          = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)FS-NL-20120731},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633) / $NFFA-Europe_supported$ -
                      Technically supported by Nanoscience Foundries and Fine
                      Analysis Europe $(2020_Join2-NFFA-Europe_funded)$},
      pid          = {G:(DE-HGF)POF4-633 /
                      $G:(DE-HGF)2020_Join2-NFFA-Europe_funded$},
      experiment   = {EXP:(DE-H253)Nanolab-04-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34355148},
      UT           = {WOS:000677580600110},
      doi          = {10.1016/j.isci.2021.102818},
      url          = {https://bib-pubdb1.desy.de/record/473889},
}