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000473295 041__ $$aEnglish
000473295 1001_ $$0P:(DE-HGF)0$$aLupp, Kilian$$b0$$eCorresponding author$$gmale
000473295 245__ $$a3D image analysis of the morphology and the oxygen diffusion within microparticle-enhanced fungal pellets using synchrotron radiation-based X-raymicrotomography $$f2020-10-19 - 2021-09-18
000473295 260__ $$bTechnische Universität München, School of Life Sciences$$c2021
000473295 300__ $$a130
000473295 3367_ $$2DRIVER$$abachelorThesis
000473295 3367_ $$02$$2EndNote$$aThesis
000473295 3367_ $$2DataCite$$aOutput Types/Supervised Student Publication
000473295 3367_ $$0PUB:(DE-HGF)2$$2PUB:(DE-HGF)$$aBachelor Thesis$$bbachelor$$mbachelor$$s1676464948_2624
000473295 3367_ $$2BibTeX$$aMASTERSTHESIS
000473295 3367_ $$2ORCID$$aSUPERVISED_STUDENT_PUBLICATION
000473295 502__ $$aBachelorarbeit, Technische Universität München, 2021$$bBachelorarbeit$$cTechnische Universität München$$d2021$$o2021-09-18
000473295 520__ $$aFilamentous fungi are fo high biotechnological interest. Their fermentation products can beused for producig various goods. The productivity strongly depends on the fungal morphologicalcharacteristics. From a process engineering point of view, the pellet formation of filamentousfungi shows several advantages due to Newtonian behavior of the fermentationbroth. Furthermore, the production of certain bioproducts, e.g. citric acid is enhanced in fungalpellets. However, this densely packed morphological form provides certain biochemicaldisadvantages. Oxygen limitation may cause inactivation of fungal biomass. Therefore, growingsmall, uniform pellets is desired. This can be gained via various morphological engineeringtools, whereas microparticle-enhanced cultivation (MPEC) has been established as a flexibleand reproducible method. For the investigation of morphological characteristics and substratelimitations in the pellet’s interior, detailed knowledge of the three-dimensional morphology isrequired.in this thesis synchrotron radiation based X-ray microtomography was used to create detailed3D images of fungal pellets. The image analysis and segmentation methods allow a detailedcomparison of morphological properties in connection with different micorparticle concentrations.Furthermore, required pellet parameters as the hyphal length concentration, tip concentrationand effective diffusion coefficients were interpolated to simulate the oxygen diffusionwithin pellets cultvated with MPEC. The results of the pellets’ morphological characteristicsand the simulation of oxygen diffusion in the Aspergillus niger pellets demonstrate the advantagesof MPEC and image analysis based on μCT-measurements.
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000473295 7001_ $$0P:(DE-H253)PIP1092635$$aMueller, Henri$$b1$$eThesis advisor
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000473295 9141_ $$y2021
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000473295 9201_ $$0I:(DE-588b)36241-4$$kTUM$$lTechnische Universität München$$x1
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000473295 980__ $$abachelor
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