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@PHDTHESIS{Flenner:471334,
author = {Flenner, Silja},
othercontributors = {Mueller, Martin and Salditt, Tim},
title = {{I}mplementation of {P}hase {C}ontrast {M}ethods at the
{P}05 {N}anotomography {E}ndstation at {PETRA} {III}:
{E}nabling in situ experiments},
school = {CAU Kiel},
type = {Dissertation},
reportid = {PUBDB-2021-04377},
pages = {139},
year = {2021},
note = {Dissertation, CAU Kiel, 2021},
abstract = {Hard X-ray full-field nanotomography is an ideal technique
to study the inner structureof materials non-destructively
at high spatial resolution, covering research areas suchas
material science, biology and medical research. In
particular, in situ experiments areof high importance to
study dynamics and processes in materials. High X-ray
energieshave the advantage to reduce the dose at the sample
and in the case of a transmissionX-ray microscope (TXM), to
increase the focal depth. One disadvantage of higherphoton
energies however is the low absorption contrast for many
materials, not onlyfor biological or biomedical specimen.
One approach to enhance the contrast is givenby phase
contrast methods.The main aim of this thesis is the
implementation of full-field phase contrast methods atthe
nanotomography endstation of the imaging beamline P05 at the
PETRA III storagering. In this context, the temporal
resolution is an important point in order to furtherreduce
the dose as well as enable in situ nanotomography
experiments. These two pointsare tackled by combining the
unique geometry of the presented beamline with
optimizedexperimental parameters and the development of new
tomographic techniques, as wellas integrating additional
post-processing steps using machine learning algorithms.One
approach to improve the contrast in a TXM is realized by
implementing Zernikephase contrast (ZPC). In addition, a new
denoising approach based on machine learningwas developed,
eliminating noise from nanotomographic data, in particular
when usingfast scanning modes. Utilizing these methods at
high temporal resolutions is key toperform the first in situ
nanotomography experiments at P05: A spider attachmenthair
is attached to a surface under force control and scanned at
different states ofattachment. An interesting aspect is here
also the orientation distribution of singleattachment
elements, which is linked to previously recorded diffraction
data.Another phase contrast approach utilizing the high
coherence of 3rd generation sources,is near-field
holotomography (NFH) based on propagation phase contrast. In
the frame-work of this thesis the worldwide first hard X-ray
holotomography setup using Fresnelzone plates has been
developed and realized at the P05 imaging beamline. In
contrastto ZPC, it offers a scalable field of view and
magnification, the quantitative analysis ofthe phase signal
and sufficient space for extended sample environments.The
developed and implemented phase contrast methods at the P05
nanotomographystation will enable the analysis of materials
with high resolution in 3D at high temporalresolutions.
Altogether this will open doors to in situ experiments and
offer greatopportunities to study dynamical processes.},
cin = {Hereon},
cid = {I:(DE-H253)Hereon-20210428},
pnm = {6G3 - PETRA III (DESY) (POF4-6G3)},
pid = {G:(DE-HGF)POF4-6G3},
experiment = {EXP:(DE-H253)P-P05-20150101},
typ = {PUB:(DE-HGF)11},
url = {https://bib-pubdb1.desy.de/record/471334},
}