000465471 001__ 465471
000465471 005__ 20250716150817.0
000465471 0247_ $$2doi$$a10.1364/OE.440173
000465471 0247_ $$2datacite_doi$$a10.3204/PUBDB-2021-03936
000465471 0247_ $$2altmetric$$aaltmetric:114679663
000465471 0247_ $$2pmid$$a34809231
000465471 0247_ $$2WOS$$aWOS:000708940500134
000465471 0247_ $$2openalex$$aopenalex:W4206282419
000465471 037__ $$aPUBDB-2021-03936
000465471 041__ $$aEnglish
000465471 082__ $$a530
000465471 1001_ $$0P:(DE-H253)PIP1020096$$aAwel, Salah$$b0$$eCorresponding author
000465471 245__ $$aOptical bunching of particles in a liquid flow
000465471 260__ $$aWashington, DC$$bSoc.$$c2021
000465471 3367_ $$2DRIVER$$aarticle
000465471 3367_ $$2DataCite$$aOutput Types/Journal article
000465471 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1718799214_3801770
000465471 3367_ $$2BibTeX$$aARTICLE
000465471 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000465471 3367_ $$00$$2EndNote$$aJournal Article
000465471 520__ $$aHigh-speed liquid micro-jets are used to rapidly and repeatedly deliver protein microcrystals to focused and pulsed X-ray beams in the method of serial femtosecond crystallography. However, the current continuous flow of crystals is mismatched to the arrival of X-ray pulses, wasting vast amounts of an often rare and precious sample. Here, we introduce a method to address this problem by periodically trapping and releasing crystals in the liquid flow, creating locally concentrated crystal bunches, using an optical trap integrated in the microfluidic supply line. We experimentally demonstrate a 30-fold increase of particle concentration into 10 Hz bunches of 6.4 μm diameter polystyrene particles. Furthermore, using particle trajectory simulations, a comprehensive description of the optical bunching process and parameter space is presented. Adding this compact optofluidics device to existing injection systems would thereby dramatically reduce sample consumption and extend the application of serial crystallography to a greater range of protein crystal systems that cannot be produced in high abundance. Our approach is suitable for other microfluidic systems that require synchronous measurements of flowing objects.
000465471 536__ $$0G:(DE-HGF)POF4-633$$a633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633)$$cPOF4-633$$fPOF IV$$x0
000465471 536__ $$0G:(DE-H253)DFG-Leibniz-2015-Chapman$$aLeibniz Preis - Leibiz Programm 2015: Prof. Dr. Henry N. Chapman (DFG-Leibniz-2015-Chapman)$$cDFG-Leibniz-2015-Chapman$$x1
000465471 536__ $$0G:(GEPRIS)390715994$$aDFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994)$$c390715994$$x2
000465471 536__ $$0G:(GEPRIS)194651731$$aDFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731)$$c194651731$$x3
000465471 536__ $$0G:(DE-HGF)2020_InternLabs-0011$$aInternLabs-0011 - HIR3X - Helmholtz International Laboratory on Reliability, Repetition, Results at the most advanced X-ray Sources (2020_InternLabs-0011)$$c2020_InternLabs-0011$$x4
000465471 588__ $$aDataset connected to CrossRef, Journals: bib-pubdb1.desy.de
000465471 693__ $$0EXP:(DE-H253)CFEL-Exp-20150101$$5EXP:(DE-H253)CFEL-Exp-20150101$$eExperiments at CFEL$$x0
000465471 7001_ $$0P:(DE-H253)PIP1080334$$aBohne, Sven$$b1
000465471 7001_ $$0P:(DE-H253)PIP1093973$$aEbrahimifard, Reza$$b2
000465471 7001_ $$0P:(DE-HGF)0$$aTrieu, Hoc Khiem$$b3
000465471 7001_ $$0P:(DE-H253)PIP1006443$$aBajt, Sasa$$b4
000465471 7001_ $$0P:(DE-H253)PIP1006324$$aChapman, Henry N.$$b5
000465471 773__ $$0PERI:(DE-600)1491859-6$$a10.1364/OE.440173$$gVol. 29, no. 21, p. 34394 -$$n21$$p34394 - 34410$$tOptics express$$v29$$x1094-4087$$y2021
000465471 8564_ $$uhttps://doi.org/10.1364/OE.440173
000465471 8564_ $$uhttps://bib-pubdb1.desy.de/record/465471/files/Admin-Chapman.pdf
000465471 8564_ $$uhttps://bib-pubdb1.desy.de/record/465471/files/HTML-Approval_of_scientific_publication.html
000465471 8564_ $$uhttps://bib-pubdb1.desy.de/record/465471/files/PDF-Approval_of_scientific_publication.pdf
000465471 8564_ $$uhttps://bib-pubdb1.desy.de/record/465471/files/Admin-Chapman.pdf?subformat=pdfa$$xpdfa
000465471 8564_ $$uhttps://bib-pubdb1.desy.de/record/465471/files/Awel2021-submitted.pdf$$yOpenAccess
000465471 8564_ $$uhttps://bib-pubdb1.desy.de/record/465471/files/publisher%27s%20version.pdf$$yOpenAccess
000465471 8564_ $$uhttps://bib-pubdb1.desy.de/record/465471/files/Awel2021-submitted.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000465471 8564_ $$uhttps://bib-pubdb1.desy.de/record/465471/files/publisher%27s%20version.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000465471 8767_ $$81577758$$92021-10-06$$d2021-10-06$$eHybrid-OA$$jZahlung erfolgt$$lMasterCard$$p440173$$zpublisher´s own licence
000465471 8767_ $$d2021-11-04$$eOther$$jZahlung erfolgt$$lMasterCard$$z1,5% Währungsumrechnung
000465471 909CO $$ooai:bib-pubdb1.desy.de:465471$$pdnbdelivery$$popenCost$$pVDB$$pdriver$$pOpenAPC$$popen_access$$popenaire
000465471 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1020096$$aDeutsches Elektronen-Synchrotron$$b0$$kDESY
000465471 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1020096$$aCentre for Free-Electron Laser Science$$b0$$kCFEL
000465471 9101_ $$0I:(DE-588)1043621512$$6P:(DE-H253)PIP1020096$$aEuropean XFEL$$b0$$kXFEL.EU
000465471 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1080334$$aExternal Institute$$b1$$kExtern
000465471 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1093973$$aDeutsches Elektronen-Synchrotron$$b2$$kDESY
000465471 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1093973$$aCentre for Free-Electron Laser Science$$b2$$kCFEL
000465471 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1006443$$aDeutsches Elektronen-Synchrotron$$b4$$kDESY
000465471 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1006443$$aCentre for Free-Electron Laser Science$$b4$$kCFEL
000465471 9101_ $$0I:(DE-588)1043621512$$6P:(DE-H253)PIP1006443$$aEuropean XFEL$$b4$$kXFEL.EU
000465471 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1006324$$aDeutsches Elektronen-Synchrotron$$b5$$kDESY
000465471 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1006324$$aCentre for Free-Electron Laser Science$$b5$$kCFEL
000465471 9101_ $$0I:(DE-588)1043621512$$6P:(DE-H253)PIP1006324$$aEuropean XFEL$$b5$$kXFEL.EU
000465471 9131_ $$0G:(DE-HGF)POF4-633$$1G:(DE-HGF)POF4-630$$2G:(DE-HGF)POF4-600$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vLife Sciences – Building Blocks of Life: Structure and Function$$x0
000465471 9141_ $$y2021
000465471 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-01-28
000465471 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000465471 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bOPT EXPRESS : 2019$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000465471 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Blind peer review$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2021-01-28
000465471 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2021-01-28
000465471 9201_ $$0I:(DE-H253)FS-CFEL-1-20120731$$kFS-CFEL-1$$lCFEL-Coherent X-Ray Imaging$$x0
000465471 9201_ $$0I:(DE-H253)CFEL-XOM-20160915$$kCFEL-XOM$$lFS-ML$$x1
000465471 9201_ $$0I:(DE-H253)CFEL-I-20161114$$kCFEL-I$$lFS-CFEL-1 (Group Leader: Henry Chapman)$$x2
000465471 980__ $$ajournal
000465471 980__ $$aVDB
000465471 980__ $$aI:(DE-H253)FS-CFEL-1-20120731
000465471 980__ $$aI:(DE-H253)CFEL-XOM-20160915
000465471 980__ $$aI:(DE-H253)CFEL-I-20161114
000465471 980__ $$aAPC
000465471 980__ $$aUNRESTRICTED
000465471 9801_ $$aAPC
000465471 9801_ $$aFullTexts