TY  - JOUR
AU  - Malik, Ankita
AU  - Seeberger, Peter H.
AU  - Brezesinski, Gerald
AU  - Varón Silva, Daniel
TI  - Zwitterionic Character and Lipid Composition Determine the Behaviour of Glycosylphosphatidylinositol Fragments in Monolayers
JO  - ChemPhysChem
VL  - 22
IS  - 8
SN  - 1439-7641
CY  - Weinheim
PB  - Wiley-VCH Verl.
M1  - PUBDB-2021-03780
SP  - 757 - 763
PY  - 2021
AB  - Glycosylphosphatidylinositols (GPIs) are complex glycolipids found in free form or anchoring proteins to the outer leaflet of the cell membrane in eukaryotes. GPIs have been associated with the formation of lipid rafts and protein sorting on membranes. The presence of a conserved glycan core with cell-specific modifications together with lipid remodelling during biosynthesis suggest that the properties of the glycolipids are being fine-tuned. We synthesized a series of GPI fragments and evaluated the interactions and arrangement of these glycolipids in monolayers as a 2-D membrane model. GIXD and IRRAS analyses showed the need of N-acetylglucosamine deacetylation for the formation of hydrogen bonds to obtain highly structured domains in the monolayers and an effect of the unsaturated lipids in formation and localization of the glycolipids within or between membrane microdomains. These results contribute to understand the role of these glycolipids and their modifications in the organization of membranes. 
LB  - PUB:(DE-HGF)16
C6  - pmid:33586851
UR  - <Go to ISI:>//WOS:000628883400001
DO  - DOI:10.1002/cphc.202100002
UR  - https://bib-pubdb1.desy.de/record/463474
ER  -