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@ARTICLE{Mieczkowski:462564,
      author       = {Mieczkowski, Mateusz and Steinmetzger, Christian and Bessi,
                      Irene and Lenz, Ann-Kathrin and Schmiedel, Alexander and
                      Holzapfel, Marco and Lambert, Christoph and Pena, Vladimir
                      and Hoebartner, Claudia},
      title        = {{L}arge {S}tokes shift fluorescence activation in an {RNA}
                      aptamer by intermolecular proton transfer to guanine},
      journal      = {Nature Communications},
      volume       = {12},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {PUBDB-2021-03466},
      pages        = {3549},
      year         = {2021},
      abstract     = {Fluorogenic RNA aptamers are synthetic functional RNAs that
                      specifically bind and activate conditional fluorophores. The
                      Chili RNA aptamer mimics large Stokes shift fluorescent
                      proteins and exhibits high affinity for
                      3,5-dimethoxy-4-hydroxybenzylidene imidazolone (DMHBI)
                      derivatives to elicit green or red fluorescence emission.
                      Here, we elucidate the structural and mechanistic basis of
                      fluorescence activation by crystallography and time-resolved
                      optical spectroscopy. Two co-crystal structures of the Chili
                      RNA with positively charged DMHBO+ and DMHBI+ ligands
                      revealed a G-quadruplex and a trans-sugar-sugar edge G:G
                      base pair that immobilize the ligand by π-π stacking. A
                      Watson-Crick G:C base pair in the fluorophore binding site
                      establishes a short hydrogen bond between the N7 of guanine
                      and the phenolic OH of the ligand. Ultrafast excited state
                      proton transfer (ESPT) from the neutral chromophore to the
                      RNA was found with a time constant of 130 fs and revealed
                      the mode of action of the large Stokes shift fluorogenic RNA
                      aptamer.},
      cin          = {DOOR ; HAS-User / UWuerzburg},
      ddc          = {500},
      cid          = {I:(DE-H253)HAS-User-20120731 /
                      I:(DE-H253)UWuerzburg-20140802},
      pnm          = {6G3 - PETRA III (DESY) (POF4-6G3) / FS-Proposal: I-20190778
                      (I-20190778) / FS-Proposal: I-20200799 (I-20200799)},
      pid          = {G:(DE-HGF)POF4-6G3 / G:(DE-H253)I-20190778 /
                      G:(DE-H253)I-20200799},
      experiment   = {EXP:(DE-H253)P-P11-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34112799},
      UT           = {WOS:000663750600005},
      doi          = {10.1038/s41467-021-23932-0},
      url          = {https://bib-pubdb1.desy.de/record/462564},
}