TY  - JOUR
AU  - Ishak, Mohd I.
AU  - Jenkins, Joshua
AU  - Kulkarni, Satishkumar
AU  - Keller, Thomas F.
AU  - Briscoe, Wuge
AU  - Nobbs, Angela H.
AU  - Su, Bo
TI  - Insights into complex nanopillar-bacteria interactions: Roles of nanotopography and bacterial surface proteins
JO  - Journal of colloid and interface science
VL  - 604
SN  - 0021-9797
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - PUBDB-2021-03218
SP  - 91 - 103
PY  - 2021
AB  - Nanopillared surfaces have emerged as a promising strategy to combat bacterial infections on medicaldevices. However, the mechanisms that underpin nanopillar-induced rupture of the bacterial cell membraneremain speculative. In this study, we have tested three medically relevant poly(ethylene terephthalate)(PET) nanopillared-surfaces with well-defined nanotopographies against both Gram-negative andGram-positive bacteria. Focused ion beam scanning electron microscopy (FIB-SEM) and contact mechanicsanalysis were utilised to understand the nanobiophysical response of the bacterial cell envelope to asingle nanopillar. Given their importance to bacterial adhesion, the contribution of bacterial surface proteinsto nanotopography-mediated cell envelope damage was also investigated. We found that, whilstcell envelope deformation was affected by the nanopillar tip diameter, the nanopillar density affectedbacterial metabolic activities. Moreover, three different types of bacterial cell envelope deformation wereobserved upon contact of bacteria with the nanopillared surfaces. These were attributed to bacterialresponses to cell wall stresses resulting from the high intrinsic pressure caused by the engagement ofnanopillars by bacterial surface proteins. Such influences of bacterial surface proteins on the antibacterial action of nanopillars have not been previously reported. Our findings will be valuable to the improveddesign and fabrication of effective antibacterial surfaces.
LB  - PUB:(DE-HGF)16
C6  - pmid:34265695
UR  - <Go to ISI:>//WOS:000703521300004
DO  - DOI:10.1016/j.jcis.2021.06.173
UR  - https://bib-pubdb1.desy.de/record/461989
ER  -