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@ARTICLE{Pawlowska:461764,
      author       = {Pawlowska, Dorota and Janich, Christopher and Langner,
                      Andreas and Dobner, Bodo and Wölk, Christian and
                      Brezesinski, Gerald},
      title        = {{T}he {I}mpact of {A}lkyl‐{C}hain {P}urity on
                      {L}ipid‐{B}ased {N}ucleic {A}cid {D}elivery {S}ystems –
                      {I}s the {U}tilization of {L}ipid {C}omponents with
                      {T}echnical {G}rade {J}ustified?},
      journal      = {ChemPhysChem},
      volume       = {20},
      number       = {16},
      issn         = {1439-7641},
      address      = {Weinheim},
      publisher    = {Wiley-VCH Verl.},
      reportid     = {PUBDB-2021-03059},
      pages        = {2110 - 2121},
      year         = {2019},
      abstract     = {The physicochemical properties and transfection efficacies
                      of two samples of a cationic lipid have been investigated
                      and compared in 2D (monolayers at the air/liquid interface)
                      and 3D (aqueous bulk dispersions) model systems using
                      different techniques. The samples differ only in their chain
                      composition due to the purity of the oleylamine (chain
                      precursor). Lipid 8 (using the oleylamine of technical grade
                      for cost-efficient synthesis) shows lateral phase separation
                      in the Langmuir layers. However, the amount of attached DNA,
                      determined by IRRAS, is for both samples the same. In 3D
                      systems, lipid 8 p forms cubic phases, which disappear
                      after addition of DNA. At physiological temperatures, both
                      lipids (alone and in mixture with cholesterol) assemble to
                      lamellar aggregates and exhibit comparable DNA delivery
                      efficiency. This study demonstrates that non-lamellar
                      structures are not compulsory for high transfection rates.
                      The results legitimate the utilization of oleyl chains of
                      technical grade in the synthesis of cationic transfection
                      lipids},
      cin          = {FS-PS},
      ddc          = {540},
      cid          = {I:(DE-H253)FS-PS-20131107},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      experiment   = {EXP:(DE-H253)D-A2-20150101 / EXP:(DE-H253)D-BW1-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31265754},
      UT           = {WOS:000477441000001},
      doi          = {10.1002/cphc.201900480},
      url          = {https://bib-pubdb1.desy.de/record/461764},
}