%0 Journal Article
%A Sponga, Antonio
%A Arolas, Joan L.
%A Schwarz, Thomas C.
%A Jeffries, Cy M.
%A Rodriguez Chamorro, Ariadna
%A Kostan, Julius
%A Ghisleni, Andrea
%A Drepper, Friedel
%A Polyansky, Anton
%A De Almeida Ribeiro, Euripedes
%A Pedron, Miriam
%A Zawadzka-Kazimierczuk, Anna
%A Mlynek, Georg
%A Peterbauer, Thomas
%A Doto, Pierantonio
%A Schreiner, Claudia
%A Hollerl, Eneda
%A Mateos, Borja
%A Geist, Leonhard
%A Faulkner, Georgine
%A Kozminski, Wiktor
%A Svergun, Dmitri I.
%A Warscheid, Bettina
%A Zagrovic, Bojan
%A Gautel, Mathias
%A Konrat, Robert
%A Djinović-Carugo, Kristina
%T Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin
%J Science advances
%V 7
%N 22
%@ 2375-2548
%C Washington, DC [u.a.]
%I Assoc.
%M PUBDB-2021-02832
%P eabg7653 -
%D 2021
%X In sarcomeres, α-actinin cross-links actin filaments and anchors them to the Z-disk. FATZ (filamin-, α-actinin-, and telethonin-binding protein of the Z-disk) proteins interact with α-actinin and other core Z-disk proteins, contributing to myofibril assembly and maintenance. Here, we report the first structure and its cellular validation of α-actinin-2 in complex with a Z-disk partner, FATZ-1, which is best described as a conformational ensemble. We show that FATZ-1 forms a tight fuzzy complex with α-actinin-2 and propose an interaction mechanism via main molecular recognition elements and secondary binding sites. The obtained integrative model reveals a polar architecture of the complex which, in combination with FATZ-1 multivalent scaffold function, might organize interaction partners and stabilize α-actinin-2 preferential orientation in Z-disk. Last, we uncover FATZ-1 ability to phase-separate and form biomolecular condensates with α-actinin-2, raising the question whether FATZ proteins can create an interaction hub for Z-disk proteins through membraneless compartmentalization during myofibrillogenesis.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:34049882
%U <Go to ISI:>//WOS:000655906900037
%R 10.1126/sciadv.abg7653
%U https://bib-pubdb1.desy.de/record/459946