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@ARTICLE{Osterode:456297,
author = {Osterode, Wolf and Falkenberg, Gerald and Regele, Heinz},
title = {{G}adolinium distribution in kidney tissue determined and
quantified by micro synchrotron {X}-ray fluorescence},
journal = {BioMetals},
volume = {34},
number = {2},
issn = {1572-8773},
address = {Dordrecht [u.a.]},
publisher = {Springer Science + Business Media B.V},
reportid = {PUBDB-2021-01448},
pages = {341 - 350},
year = {2021},
note = {Waiting for fulltext},
abstract = {Aims of this study were to investigate gadolinium (Gd) in
kidney tissue from a female patient with severe renal
failure, who had a magnetic resonance imaging (MRI) with
Gd-based contrast agent (GBCA) three times prior to kidney
transplantation. Secondly to assess (semi-)quantitatively
the Gd concentration in renal tissue and the spatial
distribution of Gd in association to suspected co-elements
such as calcium (Ca) and zinc (Zn). Archival paraffin
embedded kidney tissue was investigated by micro Synchrotron
X-ray fluorescence (µSRXRF) at the DORIS III storage ring
at beamline L, HASYLAB/DESY(Hamburg, Germany). Elementary
gadolinium (Gd) could be demonstrated in a near histological
resolution in areas of about 2 × 1.5 mm$^2$ of size.
Mean Gd resulted in 200 ppm with a huge width of
distribution (Gd-max: 2000 ppm). In kidney cortex Gd was
in-homogeneously, but not randomly, distributed. Gd was
verified throughout the investigated tissue. Low Gd was
predominately concentrated either in areas with focally
atrophic tubules or in areas with totally preserved
uriniferous tubes. Moreover, strong correlations existed
between Gd and calcium (Ca) or Gd and zinc (Zn) or Gd and
strontium (Sr) distribution. Throughout our analysed areas
copper (Cu) was nearly homogeneously distributed and Cu
association to Gd could not be established, and also not for
Gd to Fe. Gd in glomeruli was relatively reduced compared
with mean Gd-values, while iron (Fe) distribution clearly
demarks glomeruli mostly due to red blood cell iron in these
capillary convolutes. Quantitative µSRXRF analysis provided
an insight in element spatial distribution of Gd in the
renal cortex. The strong correlation of the spatial
distribution and associations between elements like Ca, Zn
and Sr let us suspect that these elements are involved in
the cell metabolism of GBCA. Low Gd in areas with extreme
fibrosis and tubule atrophy or in areas with histologically
intact tubes, let us suspect that on the one side Gd cannot
be transported and deposited into these tissue areas and on
the other side we assume that intact renal tubes do not
reabsorb and store excreted Gd.},
cin = {FS-PET-S},
ddc = {540},
cid = {I:(DE-H253)FS-PET-S-20190712},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633)},
pid = {G:(DE-HGF)POF4-633},
experiment = {EXP:(DE-H253)D-L-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33486677},
UT = {WOS:000610479300002},
doi = {10.1007/s10534-020-00284-8},
url = {https://bib-pubdb1.desy.de/record/456297},
}