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@ARTICLE{Krichel:454611,
      author       = {Krichel, Boris and Falke, Sven and Hilgenfeld, Rolf and
                      Redecke, Lars and Uetrecht, Charlotte},
      title        = {{P}rocessing of the {SARS}-{C}o{V} pp1a/ab nsp7–10
                      region},
      journal      = {The biochemical journal / Reviews},
      volume       = {477},
      number       = {5},
      issn         = {1470-8728},
      address      = {London [u.a.]},
      publisher    = {Portland Pr.8686},
      reportid     = {PUBDB-2021-00613},
      pages        = {1009 - 1019},
      year         = {2020},
      abstract     = {Severe acute respiratory syndrome coronavirus is the
                      causative agent of a respiratory disease with a high case
                      fatality rate. During the formation of the coronaviral
                      replication/transcription complex, essential steps include
                      processing of the conserved polyprotein nsp7–10 region by
                      the main protease M$^{pro}$ and subsequent complex formation
                      of the released nsp's. Here, we analyzed processing of the
                      coronavirus nsp7–10 region using native mass spectrometry
                      showing consumption of substrate, rise and fall of
                      intermediate products and complexation. Importantly, there
                      is a clear order of cleavage efficiencies, which is
                      influenced by the polyprotein tertiary structure.
                      Furthermore, the predominant product is an nsp7+8(2 : 2)
                      hetero-tetramer with nsp8 scaffold. In conclusion, native
                      MS, opposed to other methods, can expose the processing
                      dynamics of viral polyproteins and the landscape of protein
                      interactions in one set of experiments. Thereby, new
                      insights into protein interactions, essential for generation
                      of viral progeny, were provided, with relevance for
                      development of antivirals.},
      cin          = {FS-PS / DOOR ; HAS-User / U Lübeck},
      ddc          = {540},
      cid          = {I:(DE-H253)FS-PS-20131107 / I:(DE-H253)HAS-User-20120731 /
                      $I:(DE-H253)U_L__beck-20211012$},
      pnm          = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
                      633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633)},
      pid          = {G:(DE-HGF)POF3-6215 / G:(DE-HGF)POF4-633},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32083638},
      UT           = {WOS:000523312700002},
      doi          = {10.1042/BCJ20200029},
      url          = {https://bib-pubdb1.desy.de/record/454611},
}