TY  - JOUR
AU  - Eckermann, Marina
AU  - Frohn, Jasper
AU  - Reichardt, Marius
AU  - Osterhoff, Markus
AU  - Sprung, Michael
AU  - Westermeier, Fabian
AU  - Tzankov, Alexandar
AU  - Werlein, Christopher
AU  - Kühnel, Mark
AU  - Jonigk, Danny
AU  - Salditt, Tim
TI  - 3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography
JO  - eLife
VL  - 9
SN  - 2050-084X
CY  - Cambridge
PB  - eLife Sciences Publications
M1  - PUBDB-2021-00594
SP  - e60408 (1-25)
PY  - 2020
AB  - We present a three-dimensional (3D) approach for virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we propose multi-scale phase contrast x-ray tomography as a tool to unravel the pathophysiology of Covid-19, extending conventional histology by a third dimension and allowing for full quantification of tissue remodeling. By combining parallel and cone beam geometry, autopsy samples with a maximum cross section of 8 mm are scanned and reconstructed at a resolution and image quality, which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry, regions-of-interest are reconstructed with a minimum voxel size of 167 nm. We exemplify the capability of this approach by 3D visualization of diffuse alveolar damage (DAD) with its prominent hyaline membrane formation, by mapping the 3D distribution and density of lymphocytes infiltrating the tissue, and by providing histograms of characteristic distances from tissue interior to the closest air compartment.
LB  - PUB:(DE-HGF)16
C6  - pmid:32815517
UR  - <Go to ISI:>//WOS:000569336800001
DO  - DOI:10.7554/eLife.60408
UR  - https://bib-pubdb1.desy.de/record/454543
ER  -