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@ARTICLE{Salinas:454475,
author = {Salinas, Nir and Tayeb-Fligelman, Einav and Sammito,
Massimo D. and Bloch, Daniel and Jelinek, Raz and Noy, Dror
and Usón, Isabel and Landau, Meytal},
title = {{T}he amphibian antimicrobial peptide uperin 3.5 is a
cross-α/cross-β chameleon functional amyloid},
journal = {Proceedings of the National Academy of Sciences of the
United States of America},
volume = {118},
number = {3},
issn = {1091-6490},
address = {Washington, DC},
publisher = {National Acad. of Sciences},
reportid = {PUBDB-2021-00549},
pages = {e2014442118 (1-8)},
year = {2021},
abstract = {Antimicrobial activity is being increasingly linked to
amyloid fibril formation, suggesting physiological roles for
some human amyloids, which have historically been viewed as
strictly pathological agents. This work reports on formation
of functional cross-α amyloid fibrils of the amphibian
antimicrobial peptide uperin 3.5 at atomic resolution, an
architecture initially discovered in the bacterial PSMα3
cytotoxin. The fibrils of uperin 3.5 and PSMα3 comprised
antiparallel and parallel helical sheets, respectively,
recapitulating properties of β-sheets. Uperin 3.5
demonstrated chameleon properties of a secondary structure
switch, forming mostly cross-β fibrils in the absence of
lipids. Uperin 3.5 helical fibril formation was largely
induced by, and formed on, bacterial cells or membrane
mimetics, and led to membrane damage and cell death. These
findings suggest a regulation mechanism, which includes
storage of inactive peptides as well as environmentally
induced activation of uperin 3.5, via chameleon cross-α/β
amyloid fibrils.},
cin = {EMBL-User / CSSB-EMBL / CSSB-F},
ddc = {500},
cid = {I:(DE-H253)EMBL-User-20120814 /
I:(DE-H253)CSSB-EMBL-20141216 / I:(DE-H253)CSSB-F-20230420},
pnm = {6G3 - PETRA III (DESY) (POF4-6G3)},
pid = {G:(DE-HGF)POF4-6G3},
experiment = {EXP:(DE-H253)P-P14-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33431675},
UT = {WOS:000609633900031},
doi = {10.1073/pnas.2014442118},
url = {https://bib-pubdb1.desy.de/record/454475},
}