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@INBOOK{Franke:453978,
      author       = {Franke, Daniel and Svergun, Dmitri I.},
      title        = {{S}ynchrotron {S}mall-{A}ngle {X}-{R}ay {S}cattering on
                      {B}iological {M}acromolecules in {S}olution},
      address      = {Cham},
      publisher    = {Springer International Publishing},
      reportid     = {PUBDB-2021-00335},
      pages        = {1645-1672},
      year         = {2020},
      comment      = {Synchrotron Light Sources and Free-Electron Lasers /
                      Jaeschke, Eberhard J. (Editor) ; Cham : Springer
                      International Publishing, 2020, Chapter 34 ; ISBN:
                      978-3-030-23200-9 ; doi:10.1007/978-3-030-23201-6},
      booktitle     = {Synchrotron Light Sources and
                       Free-Electron Lasers / Jaeschke,
                       Eberhard J. (Editor) ; Cham : Springer
                       International Publishing, 2020, Chapter
                       34 ; ISBN: 978-3-030-23200-9 ;
                       doi:10.1007/978-3-030-23201-6},
      abstract     = {Small-angle X-ray scattering (SAXS) is a powerful method
                      for the structural analysis of macromolecular solutions,
                      allowing one to study the structure of native particles and
                      complexes and to rapidly analyze structural changes in
                      response to variations in external conditions. On
                      synchrotrons, SAXS benefits enormously from the high
                      brilliance of the radiation, providing a considerable
                      advantage for the timescale of measurements (allowing also
                      for time-resolved experiments) and the small amounts of
                      material required. Emerging automation of the scattering
                      experiment, data processing, and interpretation make
                      synchrotron solution SAXS a streamline tool for large-scale
                      structural studies in molecular biology. In the present
                      chapter, a brief account will be given of the basic
                      principles of SAXS by macromolecular solutions and of the
                      synchrotron SAXS instrumentation. The main concepts of SAXS
                      data analysis from monodisperse solutions will be considered
                      and the methods for computation of the overall structural
                      parameters and ab initio low-resolution shape
                      reconstructions will be presented. Further, approaches
                      combining SAXS with other structural, biophysical, and
                      biochemical techniques including validation of predicted or
                      experimentally obtained high-resolution models in solution
                      and identification of biologically active oligomers will be
                      considered. Modeling methods of the quaternary structure of
                      macromolecular complexes in terms of rigid body
                      movements/rotations of individual subunits or domains will
                      be reviewed. The approaches will also be considered to study
                      oligomeric mixtures and to quantitatively characterize
                      flexible macromolecular systems, including intrinsically
                      unfolded proteins. The new methodological developments in
                      SAXS will be illustrated by examples of practical
                      applications.},
      cin          = {EMBL},
      cid          = {I:(DE-H253)EMBL-20120731},
      pnm          = {6G3 - PETRA III (POF3-622)},
      pid          = {G:(DE-HGF)POF3-6G3},
      experiment   = {EXP:(DE-H253)P-P12-20150101},
      typ          = {PUB:(DE-HGF)7},
      doi          = {10.1007/978-3-030-23201-6_34},
      url          = {https://bib-pubdb1.desy.de/record/453978},
}