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@ARTICLE{Conceio:453976,
      author       = {Conceição, A. L. C. and Perlich, J. and Haas, S. and
                      Funari, S. S.},
      title        = {{SAXS}-{CT}: a nanostructure resolving microscopy for
                      macroscopic biologic specimens},
      journal      = {Biomedical physics $\&$ engineering express},
      volume       = {6},
      number       = {3},
      issn         = {2057-1976},
      address      = {Bristol},
      publisher    = {IOP Publ.},
      reportid     = {PUBDB-2021-00333},
      pages        = {035012},
      year         = {2020},
      abstract     = {SAXS-CT is an emerging powerful imaging technique which
                      bridges the gap between information retrieved from
                      high-resolution local techniques and information from
                      low-resolution, large field-of-view imaging, to determine
                      the nanostructure characteristics of well-ordered tissues,
                      e.g., mineralized collagen in bone. However, in the case of
                      soft tissues, features such as poor nanostructural
                      organization and high susceptibility to radiation-induced
                      damage limit the use of SAXS-CT. Here, by combining the
                      freeze-drying the specimen, preceded by formalin fixation,
                      with the nanostructure survey we identified and monitored
                      alterations on the hierarchical arrangement of triglycerides
                      and collagen fibrils three-dimensionally in breast tumor
                      specimens without requiring sample staining. A high density
                      of aligned collagen was observed precisely on the invasion
                      front of the breast carcinoma, showing the direction of
                      cancer spread, whereas substantial content of triglycerides
                      was identified, where the healthy tissue was located.
                      Finally, the approach developed here provides a path to
                      high-resolution nanostructural probing with a large
                      field-of-view, which was demonstrated through the
                      visualization of characteristic nanostructural arrangement
                      and quantification of content and degree of organization of
                      collagen fibrils in normal, benign and malignant human
                      breast tissue.},
      cin          = {FS-PET-D},
      ddc          = {610},
      cid          = {I:(DE-H253)FS-PET-D-20190712},
      pnm          = {6215 - Soft Matter, Health and Life Sciences (POF3-621)},
      pid          = {G:(DE-HGF)POF3-6215},
      experiment   = {EXP:(DE-H253)D-BW4-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33438657},
      UT           = {WOS:000522328700001},
      doi          = {10.1088/2057-1976/ab7cad},
      url          = {https://bib-pubdb1.desy.de/record/453976},
}