TY  - JOUR
AU  - Siewert, Christian
AU  - Haas, Heinrich
AU  - Cornet, Vera
AU  - Nogueira, Sara S.
AU  - Nawroth, Thomas
AU  - Uebbing, Lukas
AU  - Ziller, Antje
AU  - Al-Gousous, Jozef
AU  - Radulescu, Aurel
AU  - Schroer, Martin
AU  - Blanchet, Clement E.
AU  - Svergun, Dmitri I.
AU  - Radsak, Markus P.
AU  - Sahin, Ugur
AU  - Langguth, Peter
TI  - Hybrid Biopolymer and Lipid Nanoparticles with Improved Transfection Efficacy for mRNA
JO  - Cells
VL  - 9
IS  - 9
SN  - 2073-4409
CY  - Basel
PB  - MDPI
M1  - PUBDB-2020-04301
SP  - 2034 (1-19)
PY  - 2020
AB  - Hybrid nanoparticles from lipidic and polymeric components were assembled to serve as vehicles for the transfection of messenger RNA (mRNA) using different portions of the cationic lipid DOTAP (1,2-Dioleoyl-3-trimethylammonium-propane) and the cationic biopolymer protamine as model systems. Two different sequential assembly approaches in comparison with a direct single-step protocol were applied, and molecular organization in correlation with biological activity of the resulting nanoparticle systems was investigated. Differences in the structure of the nanoparticles were revealed by thorough physicochemical characterization including small angle neutron scattering (SANS), small angle X-ray scattering (SAXS), and cryogenic transmission electron microscopy (cryo-TEM). All hybrid systems, combining lipid and polymer, displayed significantly increased transfection in comparison to lipid/mRNA and polymer/mRNA particles alone. For the hybrid nanoparticles, characteristic differences regarding the internal organization, release characteristics, and activity were determined depending on the assembly route. The systems with the highest transfection efficacy were characterized by a heterogenous internal organization, accompanied by facilitated release. Such a system could be best obtained by the single step protocol, starting with a lipid and polymer mixture for nanoparticle formation.
LB  - PUB:(DE-HGF)16
C6  - pmid:32899484
UR  - <Go to ISI:>//WOS:000580699400001
DO  - DOI:10.3390/cells9092034
UR  - https://bib-pubdb1.desy.de/record/450355
ER  -