% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{LaheyRudolph:450351,
      author       = {Lahey-Rudolph, Janine Mia and Schönherr, Robert and
                      Jeffries, Cy M. and Blanchet, Clément E. and Boger, Juliane
                      and Ferreira Ramos, Ana Sofia and Riekehr, Winnie Maria and
                      Triandafillidis, Dimitris-Panagiotis and Valmas, Alexandros
                      and Margiolaki, Irene and Svergun, Dmitri and Redecke, Lars},
      title        = {{R}apid screening of in cellulo grown protein crystals via
                      a small-angle {X}-ray scattering/{X}-ray powder diffraction
                      synergistic approach},
      journal      = {Journal of applied crystallography},
      volume       = {53},
      number       = {5},
      issn         = {1600-5767},
      address      = {[S.l.]},
      publisher    = {Wiley-Blackwell},
      reportid     = {PUBDB-2020-04297},
      pages        = {1169 - 1180},
      year         = {2020},
      abstract     = {Crystallization of recombinant proteins in living cells is
                      an exciting new approach for structural biology that
                      provides an alternative to the time-consuming optimization
                      of protein purification and extensive crystal screening
                      steps. Exploiting the potential of this approach requires a
                      more detailed understanding of the cellular processes
                      involved and versatile screening strategies for crystals in
                      a cell culture. Particularly if the target protein forms
                      crystalline structures of unknown morphology only in a small
                      fraction of cells, their detection by applying standard
                      visualization techniques can be time consuming and difficult
                      owing to the environmental challenges imposed by the living
                      cells. In this study, a high-brilliance and low-background
                      bioSAXS beamline is employed for rapid and sensitive
                      detection of protein microcrystals grown within insect
                      cells. On the basis of the presence of Bragg peaks in the
                      recorded small-angle X-ray scattering profiles, it is
                      possible to assess within seconds whether a cell culture
                      contains microcrystals, even in a small percentage of cells.
                      Since such information cannot be obtained by other
                      established detection methods in this time frame, this
                      screening approach has the potential to overcome one of the
                      bottlenecks of intracellular crystal detection. Moreover,
                      the association of the Bragg peak positions in the
                      scattering curves with the unit-cell composition of the
                      protein crystals raises the possibility of investigating the
                      impact of environmental conditions on the crystal structure
                      of the intracellular protein crystals. This information
                      provides valuable insights helping to further understand the
                      in cellulo crystallization process.},
      cin          = {EMBL-User / EMBL / FS-CFEL-1 / DOOR ; HAS-User / U Lübeck},
      ddc          = {540},
      cid          = {I:(DE-H253)EMBL-User-20120814 / I:(DE-H253)EMBL-20120731 /
                      I:(DE-H253)FS-CFEL-1-20120731 / I:(DE-H253)HAS-User-20120731
                      / $I:(DE-H253)U_L__beck-20211012$},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633) / 6G3 - PETRA III (POF3-622)},
      pid          = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF3-6G3},
      experiment   = {EXP:(DE-H253)P-P12-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33117106},
      UT           = {WOS:000577178000001},
      doi          = {10.1107/S1600576720010687},
      url          = {https://bib-pubdb1.desy.de/record/450351},
}