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@ARTICLE{Lonar:442807,
      author       = {Lončar, Nikola and Rozeboom, Henriette J. and Franken,
                      Linda E. and Stuart, Marc C. A. and Fraaije, Marco W.},
      title        = {{S}tructure of a robust bacterial protein cage and its
                      application as a versatile biocatalytic platform through
                      enzyme encapsulation},
      journal      = {Biochemical and biophysical research communications},
      volume       = {529},
      number       = {3},
      issn         = {0006-291X},
      address      = {Orlando, Fla.},
      publisher    = {Academic Press},
      reportid     = {PUBDB-2020-03227},
      pages        = {548 - 553},
      year         = {2020},
      abstract     = {Using a newly discovered encapsulin from Mycolicibacterium
                      hassiacum, several biocatalysts were packaged in this robust
                      protein cage. The encapsulin was found to be easy to produce
                      as recombinant protein. Elucidation of its crystal structure
                      revealed that it is a spherical protein cage of 60 protomers
                      (diameter of 23 nm) with narrow pores. By developing an
                      effective coexpression and isolation procedure, the effect
                      of packaging a variety of biocatalysts could be evaluated.
                      It was shown that encapsulation results in a significantly
                      higher stability of the biocatalysts. Most of the targeted
                      cofactor-containing biocatalysts remained active in the
                      encapsulin. Due to the restricted diameters of the
                      encapsulin pores (5–9 Å), the protein cage protects the
                      encapsulated enzymes from bulky compounds. The work shows
                      that encapsulins may be valuable tools to tune the
                      properties of biocatalysts such as stability and substrate
                      specificity.},
      cin          = {EMBL-User},
      ddc          = {570},
      cid          = {I:(DE-H253)EMBL-User-20120814},
      pnm          = {6G3 - PETRA III (POF3-622)},
      pid          = {G:(DE-HGF)POF3-6G3},
      experiment   = {EXP:(DE-H253)P-P13-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32736672},
      UT           = {WOS:000555780500005},
      doi          = {10.1016/j.bbrc.2020.06.059},
      url          = {https://bib-pubdb1.desy.de/record/442807},
}