TY  - JOUR
AU  - Gisriel, Chris
AU  - Coe, Jesse
AU  - Letrun, Romain
AU  - Yefanov, Oleksandr
AU  - Luna-Chavez, Cesar
AU  - Stander, Natasha E.
AU  - Lisova, Stella
AU  - Mariani, Valerio
AU  - Kuhn, Manuela
AU  - Aplin, Steve
AU  - Grant, Thomas
AU  - Dörner, Katerina
AU  - Sato, Tokushi
AU  - Echelmeier, Austin
AU  - Cruz Villarreal, Jorvani
AU  - Hunter, Mark S.
AU  - Wiedorn, Max O.
AU  - Knoska, Juraj
AU  - Mazalova, Victoria
AU  - Roy-Chowdhury, Shatabdi
AU  - Yang, Jay-How
AU  - Jones, Alex
AU  - Bean, Richard
AU  - Bielecki, Johan
AU  - Kim, Yoonhee
AU  - Mills, Grant
AU  - Weinhausen, Britta
AU  - Meza, Jose D.
AU  - Al-Qudami, Nasser
AU  - Bajt, Saša
AU  - Brehm, Gerrit
AU  - Botha, Sabine
AU  - Boukhelef, Djelloul
AU  - Brockhauser, Sandor
AU  - Bruce, Barry
AU  - Coleman, Matthew A.
AU  - Danilevski, Cyril
AU  - Discianno, Erin
AU  - Dobson, Zachary
AU  - Fangohr, Hans
AU  - Martin-Garcia, Jose M.
AU  - Gevorkov, Yaroslav
AU  - Hauf, Steffen
AU  - Hosseinizadeh, Ahmad
AU  - Januschek, Friederike
AU  - Ketawala, Gihan K.
AU  - Kupitz, Christopher
AU  - Maia, Luis
AU  - Manetti, Maurizio
AU  - Messerschmidt, Marc
AU  - Michelat, Thomas
AU  - Mondal, Jyotirmoy
AU  - Ourmazd, Abbas
AU  - Previtali, Gianpietro
AU  - Sarrou, Iosifina
AU  - Schön, Silvan
AU  - Schwander, Peter
AU  - Shelby, Megan L.
AU  - Silenzi, Alessandro
AU  - Sztuk-Dambietz, Jolanta
AU  - Szuba, Janusz
AU  - Turcato, Monica
AU  - White, Thomas
AU  - Wrona, Krzysztof
AU  - Xu, Chen
AU  - Abdellatif, Mohamed H.
AU  - Zook, James D.
AU  - Spence, John C. H.
AU  - Chapman, Henry N.
AU  - Barty, Anton
AU  - Kirian, Richard
AU  - Frank, Matthias
AU  - Ros, Alexandra
AU  - Schmidt, Marius
AU  - Fromme, Raimund
AU  - Mancuso, Adrian P.
AU  - Fromme, Petra
AU  - Zatsepin, Nadia A.
TI  - Membrane protein megahertz crystallography at the European XFEL
JO  - Nature Communications
VL  - 10
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - PUBDB-2019-03909
SP  - 11
PY  - 2019
N1  - Open access
AB  - The world’s first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid pulse rate, the European XFEL may alleviate some of the increasing demand for XFEL beamtime, particularly for membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report the first membrane protein megahertz SFX experiment, where we determined a 2.9 Å-resolution SFX structure of the large membrane protein complex, Photosystem I, a > 1 MDa complex containing 36 protein subunits and 381 cofactors. We address challenges to megahertz SFX for membrane protein complexes, including growth of large quantities of crystals and the large molecular and unit cell size that influence data collection and analysis. The results imply that megahertz crystallography could have an important impact on structure determination of large protein complexes with XFELs.
LB  - PUB:(DE-HGF)16
C6  - pmid:31685819
UR  - <Go to ISI:>//WOS:000493890900004
DO  - DOI:10.1038/s41467-019-12955-3
UR  - https://bib-pubdb1.desy.de/record/426811
ER  -