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@ARTICLE{Nicolas:420674,
author = {Nicolas, Jan-David and Bernhardt, Marten and Schlick,
Susanne F. and Tiburcy, Malte and Zimmermann,
Wolfram-Hubertus and Khan, Amara and Markus, Andrea and
Alves, Frauke and Toischer, Karl and Salditt, Tim},
title = {{X}-ray diffraction imaging of cardiac cells and tissue},
journal = {Progress in biophysics $\&$ molecular biology},
volume = {144},
issn = {0079-6107},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {PUBDB-2019-01625},
pages = {151 - 165},
year = {2019},
abstract = {With the development of advanced focusing optics for
x-rays, we can now use x-ray beams with spot sizes in the
micro- or nanometer range to scan cells and large areas of
tissues and continuously record the diffraction signals.
From this data, x-ray scattering maps or so-called x-ray
darkfield images are computed showing how different types of
cells or regions of tissues differ in their diffraction
intensity. At the same time a diffraction pattern is
available for each scan point which encodes the local
nanostructure, averaged over many contributing constituents
illuminated by the beam. In this work we have exploited
these new capabilities of scanning x-ray diffraction to
investigate cardiac muscle cells as well as cardiac tissue.
We give examples of how cardiac cells, especially living,
cultured cells, can be prepared to be compatible with the
instrumentation constraints of nano- or micro-diffraction
instruments. Furthermore, we show how the developmental
stage, ranging from neonatal to adult cells, as well as the
final preparation state of the cardiomyocytes influences the
recorded scattering signal and how these diffraction signals
compare to the structure of a fully developed cardiac
muscle.},
cin = {DOOR},
ddc = {570},
cid = {I:(DE-H253)HAS-User-20120731},
pnm = {6G3 - PETRA III (POF3-622)},
pid = {G:(DE-HGF)POF3-6G3},
experiment = {EXP:(DE-H253)P-P10-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29914693},
UT = {WOS:000472812100014},
doi = {10.1016/j.pbiomolbio.2018.05.012},
url = {https://bib-pubdb1.desy.de/record/420674},
}