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@ARTICLE{Ziller:418120,
      author       = {Ziller, Antje and Nogueira, Sara S. and Hühn, Eva and
                      Funari, Sergio de Souza and Brezesinski, Gerald and
                      Hartmann, Hermann and Sahin, Ugur and Haas, Heinrich and
                      Langguth, Peter},
      title        = {{I}ncorporation of m{RNA} in {L}amellar {L}ipid {M}atrices
                      for {P}arenteral {A}dministration},
      journal      = {Molecular pharmaceutics},
      volume       = {15},
      number       = {2},
      issn         = {1543-8392},
      address      = {Washington, DC},
      publisher    = {American Chemical Society},
      reportid     = {PUBDB-2019-00207},
      pages        = {642 - 651},
      year         = {2018},
      note         = {© American Chemical Society},
      abstract     = {Insertion of high molecular weight messenger RNA (mRNA)
                      into lyotropic lipid phases as model systems for controlled
                      release formulations for the mRNA was investigated. Low
                      fractions of 1,2-dioleoyl-3-trimethylammonium-propane
                      (DOTAP) were used as an anchor to load the mRNA into a
                      lamellar lipid matrix. Dispersions of zwitterionic lipid in
                      the aqueous phase in the presence of increasing fractions of
                      mRNA and cationic lipid were prepared, and the molecular
                      organization was investigated as a function of mRNA and
                      cationic lipid fraction. Insertion of both cationic lipid
                      and mRNA was clearly proven from the physicochemical
                      characteristics. The d-spacing of the lipid bilayers, as
                      determined by small-angle X-ray scattering (SAXS)
                      measurements, responded sensitively to the amount of
                      inserted DOTAP and mRNA. A concise model of the insertion of
                      the mRNA in the lipid matrices was derived, indicating that
                      the mRNA was accommodated in the aqueous slab between lipid
                      bilayers. Depending on the DOTAP and mRNA fraction, a
                      different excess of water was present in this slab. Results
                      from further physicochemical characterization, including
                      determination of free and bound mRNA, zeta potential, and
                      calorimetry data, were in line with this assumption. The
                      structure of these concentrated lipid/mRNA preparations was
                      maintained upon dilution. The functionality of the inserted
                      mRNA was proven by cell culture experiments using C2C12
                      murine myoblast cells with the luciferase-encoding mRNA. The
                      described lipid phases as carriers for the mRNA may be
                      applicable for different routes of local administration,
                      where control of the release kinetics and the form of the
                      released mRNA (bound or free) is required.},
      cin          = {DOOR / FS-PEX},
      ddc          = {610},
      cid          = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)FS-PEX-20130206},
      pnm          = {6215 - Soft Matter, Health and Life Sciences (POF3-621)},
      pid          = {G:(DE-HGF)POF3-6215},
      experiment   = {EXP:(DE-H253)D-A2-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29232147},
      UT           = {WOS:000424730900030},
      doi          = {10.1021/acs.molpharmaceut.7b01022},
      url          = {https://bib-pubdb1.desy.de/record/418120},
}