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@ARTICLE{Kooshapur:417862,
author = {Kooshapur, Hamed and Choudhury, Nila Roy and Simon, Bernd
and Mühlbauer, Max and Jussupow, Alexander and Fernandez,
Noemi and Jones, Alisha N. and Dallmann, Andre and Gabel,
Frank and Camilloni, Carlo and Michlewski, Gracjan and
Caceres, Javier F. and Sattler, Michael},
title = {{S}tructural basis for terminal loop recognition and
stimulation of pri-mi{RNA}-18a processing by hn{RNP} {A}1},
journal = {Nature Communications},
volume = {9},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Nature Publ. Group},
reportid = {PUBDB-2019-00038},
pages = {2479},
year = {2018},
abstract = {Post-transcriptional mechanisms play a predominant role in
the control of microRNA (miRNA) production. Recognition of
the terminal loop of precursor miRNAs by RNA-binding
proteins (RBPs) influences their processing; however, the
mechanistic basis for how levels of individual or subsets of
miRNAs are regulated is mostly unexplored. We previously
showed that hnRNP A1, an RBP implicated in many aspects of
RNA processing, acts as an auxiliary factor that promotes
the Microprocessor-mediated processing of pri-mir-18a. Here,
by using an integrative structural biology approach, we show
that hnRNP A1 forms a 1:1 complex with pri-mir-18a where
both RNA recognition motifs (RRMs) bind to cognate RNA
sequence motifs in the terminal loop of pri-mir-18a.
Terminal loop binding induces an allosteric destabilization
of base-pairing in the pri-mir-18a stem that promotes its
downstream processing. Our results highlight terminal loop
RNA recognition by RBPs as a potential general principle of
miRNA biogenesis and regulation.},
cin = {EMBL-User},
ddc = {500},
cid = {I:(DE-H253)EMBL-User-20120814},
pnm = {899 - ohne Topic (POF3-899)},
pid = {G:(DE-HGF)POF3-899},
experiment = {EXP:(DE-H253)D-D1.2-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29946118},
UT = {WOS:000436236800007},
doi = {10.1038/s41467-018-04871-9},
url = {https://bib-pubdb1.desy.de/record/417862},
}