TY  - JOUR
AU  - Kooshapur, Hamed
AU  - Choudhury, Nila Roy
AU  - Simon, Bernd
AU  - Mühlbauer, Max
AU  - Jussupow, Alexander
AU  - Fernandez, Noemi
AU  - Jones, Alisha N.
AU  - Dallmann, Andre
AU  - Gabel, Frank
AU  - Camilloni, Carlo
AU  - Michlewski, Gracjan
AU  - Caceres, Javier F.
AU  - Sattler, Michael
TI  - Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1
JO  - Nature Communications
VL  - 9
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publ. Group
M1  - PUBDB-2019-00038
SP  - 2479
PY  - 2018
AB  - Post-transcriptional mechanisms play a predominant role in the control of microRNA (miRNA) production. Recognition of the terminal loop of precursor miRNAs by RNA-binding proteins (RBPs) influences their processing; however, the mechanistic basis for how levels of individual or subsets of miRNAs are regulated is mostly unexplored. We previously showed that hnRNP A1, an RBP implicated in many aspects of RNA processing, acts as an auxiliary factor that promotes the Microprocessor-mediated processing of pri-mir-18a. Here, by using an integrative structural biology approach, we show that hnRNP A1 forms a 1:1 complex with pri-mir-18a where both RNA recognition motifs (RRMs) bind to cognate RNA sequence motifs in the terminal loop of pri-mir-18a. Terminal loop binding induces an allosteric destabilization of base-pairing in the pri-mir-18a stem that promotes its downstream processing. Our results highlight terminal loop RNA recognition by RBPs as a potential general principle of miRNA biogenesis and regulation. 
LB  - PUB:(DE-HGF)16
C6  - pmid:29946118
UR  - <Go to ISI:>//WOS:000436236800007
DO  - DOI:10.1038/s41467-018-04871-9
UR  - https://bib-pubdb1.desy.de/record/417862
ER  -