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@INPROCEEDINGS{Chia:415778,
author = {Chia, Shih-Hsuan and Chung, Hsiang-Yu and Liu, Wei and
Kärtner, Franz and Chang, Guoqing},
title = {{D}eep {T}issue {M}ultiphoton {M}icroscopy {B}ased on
{A}dvanced {F}emtosecond {F}iber {S}ources},
address = {[Piscataway, NJ]},
publisher = {IEEE},
reportid = {PUBDB-2018-04681},
isbn = {978-1-5386-6605-0},
pages = {4},
year = {2018},
note = {IEEE},
comment = {2018 20th International Conference on Transparent Optical
Networks (ICTON) : [Proceedings] - IEEE, 2018. - ISBN
978-1-5386-6605-0 - doi:10.1109/ICTON.2018.8474004},
booktitle = {2018 20th International Conference on
Transparent Optical Networks (ICTON) :
[Proceedings] - IEEE, 2018. - ISBN
978-1-5386-6605-0 -
doi:10.1109/ICTON.2018.8474004},
abstract = {The use of multiphoton microscopy has proven its potential
on deep tissue virtual biopsy for early diagnosis and
neuronal processes imaging. However, the capability of
multiphoton microscopy strongly depends on the availability
of easily operated femtosecond laser sources. By precise
management of fiber-optic nonlinearity, we have demonstrated
the way to generate nearly transform-limited femtosecond
sources with the tunable wavelength range of 0.9 μm - 1.2
μm, covering both the GFP two-photon-excitation band and
transparency window of common bio-tissues. The demonstrated
approach is fully energy scalable to sub-100 nJ level of
pulse energy with the spectral tunable range down to 1.31
μm, which paves the way to capture deep-tissue imaging
contrast from third harmonic generation and
three-photon-excited GFP fluorescence. We applied such an
energetic source to a proof-of-principle study of ex vivo
human skin based on harmonics (i.e., second-harmonic
generation and third-harmonic generation) imaging. This new
type of fiber-format energetic ultrafast source will be
performed as a robust solution for multiphoton microscopy
applications, as well as studying the damage assessments
from thermal heating and multiphoton absorption during the
imaging process.},
month = {Jul},
date = {2018-07-01},
organization = {20th International Conference on
Transparent Optical Networks, Bucharest
(Romania), 1 Jul 2018 - 5 Jul 2018},
cin = {CFEL-ULOCM / FS-CFEL-2 / UNI/CUI},
cid = {I:(DE-H253)CFEL-ULOCM-20160928 /
I:(DE-H253)FS-CFEL-2-20120731 /
$I:(DE-H253)UNI_CUI-20121230$},
pnm = {6211 - Extreme States of Matter: From Cold Ions to Hot
Plasmas (POF3-621) / CUI - Hamburger Zentrum für
ultraschnelle Beobachtung (194651731)},
pid = {G:(DE-HGF)POF3-6211 / G:(GEPRIS)194651731},
experiment = {EXP:(DE-H253)CFEL-Exp-20150101},
typ = {PUB:(DE-HGF)8 / PUB:(DE-HGF)7},
doi = {10.1109/ICTON.2018.8474004},
url = {https://bib-pubdb1.desy.de/record/415778},
}