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@ARTICLE{Osterode:414312,
      author       = {Osterode, W. and Falkenberg, G. and Ferenci, P. and Wrba,
                      F.},
      title        = {{Q}uantitative trace element mapping in liver tissue from
                      patients with {W}ilson`s disease determined by micro {X}-ray
                      fluorescence},
      journal      = {Journal of trace elements in medicine and biology},
      volume       = {51},
      issn         = {0946-672X},
      address      = {München},
      publisher    = {Elsevier},
      reportid     = {PUBDB-2018-03866},
      pages        = {42 - 49},
      year         = {2019},
      note         = {© Elsevier GmbH.},
      abstract     = {Aims: of this investigation were to quantify copper (Cu),
                      iron (Fe) and zinc (Zn) along with sulphur (S) andphosphorus
                      (P) in hepatocytes and connective tissue in liver section
                      from patients with Wilson´s disease (WD) bymicro
                      Synchrotron X-ray fluorescence (μ-SRXRF). Secondly to
                      establish two-dimensional μ-SRXRF elementmappings for
                      comparison with histologically prepared slices, and thirdly
                      to assess whether elemental distribu-tions are
                      associated.Methods: Archival liver tissues from twelve
                      patients with end-stage cirrhosis or fulminant WD were
                      investigated.Mutations in ATP7B have been classified
                      before. For control seven archived normal liver tissues were
                      in-vestigated. μ-SRXRF measurements were performed at the
                      DORIS III storage ring at HASYLAB/DESY (Hamburg,Germany).
                      Two-dimensional element distribution were compared with
                      histologically prepared slices about20–30 μm apart from
                      those investigated by μ-SRXRF.Results: Elementary copper
                      (Cu) could be demonstrated in all investigated liver
                      sections simultaneously with Fe,Zn, P and S. In WD mean Cu
                      was 20 fold increased in hepatocytes and threefold in
                      fibrotic areas in comparisonwith controls. In regeneration
                      nodules Cu was strikingly inhomogeneous distributed. Cu
                      concentrations mea-sured by μ-SRXRF correlated with those
                      measured by atom absorption spectroscopy. Strong
                      associations in theirregional distribution existed between
                      Zn and Cu or Fe and S. Moreover, differences in Cu/S were
                      found betweenhepatocytes and fibrotic areas. An increase of
                      Fe could only be documented in hepatocytes compared to
                      fibroticareas. With a beam size of 15 x 15 μm
                      two-dimensional distributions of these elements are
                      morphologicallycomparable with histological section with a
                      magnification of about 25x optic microscope.Conclusions:
                      μ-SRXRF investigations are a valuable tool for quantifying
                      element concentrations in biologicaltissues and further
                      provide 2-dimensional information of element distribution
                      and elemental association in abiological tissues, thus
                      speeding up basic knowledge in a synopsis with biological
                      and clinical data.},
      cin          = {DOOR / FS-PE},
      ddc          = {610},
      cid          = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)FS-PE-20120731},
      pnm          = {6215 - Soft Matter, Health and Life Sciences (POF3-621)},
      pid          = {G:(DE-HGF)POF3-6215},
      experiment   = {EXP:(DE-H253)D-L-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30466937},
      UT           = {WOS:000463119100008},
      doi          = {10.1016/j.jtemb.2018.09.007},
      url          = {https://bib-pubdb1.desy.de/record/414312},
}