Home > Publications database > Structural insights into K48-linked ubiquitin chain formation by the Pex4p-Pex22p complex > print

001     399602
005     20250730105140.0
024 7 _ |a 10.1016/j.bbrc.2017.12.150
|2 doi
024 7 _ |a 0006-291X
|2 ISSN
024 7 _ |a 1090-2104
|2 ISSN
024 7 _ |a 10.3204/PUBDB-2018-00778
|2 datacite_doi
024 7 _ |a altmetric:31101901
|2 altmetric
024 7 _ |a pmid:29288668
|2 pmid
024 7 _ |a WOS:000424313500048
|2 WOS
024 7 _ |a openalex:W2780782779
|2 openalex
037 _ _ |a PUBDB-2018-00778
041 _ _ |a English
082 _ _ |a 570
100 1 _ |a Groves, Matthew R.
|0 0000-0001-9859-5177
|b 0
245 _ _ |a Structural insights into K48-linked ubiquitin chain formation by the Pex4p-Pex22p complex
260 _ _ |a Orlando, Fla.
|c 2017
|b Academic Press
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1520257534_20301
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a Pex4p is a peroxisomal E2 involved in ubiquitinating the conserved cysteine residue of the cycling receptor protein Pex5p. Previously, we demonstrated that Pex4p from the yeast Saccharomyces cerevisiae binds directly to the peroxisomal membrane protein Pex22p and that this interaction is vital for receptor ubiquitination. In addition, Pex22p binding allows Pex4p to specifically produce lysine 48 linked ubiquitin chains in vitro through an unknown mechanism. This activity is likely to play a role in targeting peroxisomal proteins for proteasomal degradation.Here we present the crystal structures of Pex4p alone and in complex with Pex22p from the yeast Hansenula polymorpha. Comparison of the two structures demonstrates significant differences to the active site of Pex4p upon Pex22p binding while molecular dynamics simulations suggest that Pex22p binding facilitates active site remodelling of Pex4p through an allosteric mechanism. Taken together, our data provide insights into how Pex22p binding allows Pex4p to build K48-linked Ub chains.
536 _ _ |a 6G3 - PETRA III (POF3-622)
|0 G:(DE-HGF)POF3-6G3
|c POF3-622
|f POF III
|x 0
588 _ _ |a Dataset connected to CrossRef
693 _ _ |a PETRA III
|f PETRA Beamline P11
|1 EXP:(DE-H253)PETRAIII-20150101
|0 EXP:(DE-H253)P-P11-20150101
|6 EXP:(DE-H253)P-P11-20150101
|x 0
700 1 _ |a Schroer, Carsten F. E.
|0 P:(DE-HGF)0
|b 1
700 1 _ |a Middleton, Adam J.
|0 0000-0002-6075-4061
|b 2
700 1 _ |a Lunev, Sergey
|0 0000-0001-9867-6866
|b 3
700 1 _ |a Danda, Natasha
|0 P:(DE-HGF)0
|b 4
700 1 _ |a Ali, Ameena M.
|0 P:(DE-HGF)0
|b 5
700 1 _ |a Marrink, Siewert J.
|0 P:(DE-HGF)0
|b 6
700 1 _ |a Williams, Chris
|0 0000-0003-1757-6156
|b 7
|e Corresponding author
773 _ _ |a 10.1016/j.bbrc.2017.12.150
|g p. S0006291X17325524
|0 PERI:(DE-600)1461396-7
|n 2
|p 562 - 567
|t Biochemical and biophysical research communications
|v 496
|y 2017
|x 0006-291X
856 4 _ |y OpenAccess
|u https://bib-pubdb1.desy.de/record/399602/files/1-s2.0-S0006291X17325524-main.pdf
856 4 _ |y OpenAccess
|x icon
|u https://bib-pubdb1.desy.de/record/399602/files/1-s2.0-S0006291X17325524-main.gif?subformat=icon
856 4 _ |y OpenAccess
|x icon-1440
|u https://bib-pubdb1.desy.de/record/399602/files/1-s2.0-S0006291X17325524-main.jpg?subformat=icon-1440
856 4 _ |y OpenAccess
|x icon-180
|u https://bib-pubdb1.desy.de/record/399602/files/1-s2.0-S0006291X17325524-main.jpg?subformat=icon-180
856 4 _ |y OpenAccess
|x icon-640
|u https://bib-pubdb1.desy.de/record/399602/files/1-s2.0-S0006291X17325524-main.jpg?subformat=icon-640
856 4 _ |y OpenAccess
|x pdfa
|u https://bib-pubdb1.desy.de/record/399602/files/1-s2.0-S0006291X17325524-main.pdf?subformat=pdfa
909 C O |o oai:bib-pubdb1.desy.de:399602
|p openaire
|p open_access
|p VDB
|p driver
|p dnbdelivery
913 1 _ |a DE-HGF
|b Forschungsbereich Materie
|l Von Materie zu Materialien und Leben
|1 G:(DE-HGF)POF3-620
|0 G:(DE-HGF)POF3-622
|2 G:(DE-HGF)POF3-600
|v Facility topic: Research on Matter with Brilliant Light Sources
|9 G:(DE-HGF)POF3-6G3
|x 0
|4 G:(DE-HGF)POF
|3 G:(DE-HGF)POF3
914 1 _ |y 2017
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
915 _ _ |a Creative Commons Attribution CC BY 4.0
|0 LIC:(DE-HGF)CCBY4
|2 HGFVOC
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b BIOCHEM BIOPH RES CO : 2015
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
915 _ _ |a WoS
|0 StatID:(DE-HGF)0110
|2 StatID
|b Science Citation Index
915 _ _ |a WoS
|0 StatID:(DE-HGF)0111
|2 StatID
|b Science Citation Index Expanded
915 _ _ |a IF < 5
|0 StatID:(DE-HGF)9900
|2 StatID
915 _ _ |a OpenAccess
|0 StatID:(DE-HGF)0510
|2 StatID
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0310
|2 StatID
|b NCBI Molecular Biology Database
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
915 _ _ |a Nationallizenz
|0 StatID:(DE-HGF)0420
|2 StatID
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Thomson Reuters Master Journal List
920 1 _ |0 I:(DE-H253)HAS-User-20120731
|k DOOR
|l DOOR-User
|x 0
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a UNRESTRICTED
980 _ _ |a I:(DE-H253)HAS-User-20120731
980 1 _ |a FullTexts

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21