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@ARTICLE{Mamais:397870,
author = {Mamais, Michael and DegliEsposti, Alessandra and
Kouloumoundra, Virginia and Gustavsson, Thomas and Monti,
Filippo and Venturini, Alessandro and Chrysina, Evangelia D.
and Markovitsi, Dimitra and Gimisis, Thanasis},
title = {{A} {N}ew {P}otent {I}nhibitor of {G}lycogen
{P}hosphorylase {R}eveals the {B}asicity of the {C}atalytic
{S}ite},
journal = {Chemistry - a European journal},
volume = {23},
number = {37},
issn = {0947-6539},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {PUBDB-2017-13502},
pages = {8800 - 8805},
year = {2017},
note = {© Wiley-VCH Verlag GmbH $\&$ Co. KGaA, Weinheim ; Post
referee fulltext in progress; Embargo 12 months from
publication},
abstract = {The design and synthesis of a glucose-based acridone
derivative (GLAC), a potent inhibitor of glycogen
phosphorylase (GP) are described. GLAC is the first
inhibitor of glycogen phosphorylase, the electronic
absorption properties of which are clearly distinguishable
from those of the enzyme. This allows probing subtle
interactions in the catalytic site. The GLAC absorption
spectra, associated with X-ray crystallography and quantum
chemistry calculations, reveal that part of the catalytic
site of GP behaves as a highly basic environment in which
GLAC exists as a bis-anion. This is explained by
water-bridged hydrogen-bonding interactions with specific
catalytic site residues.},
cin = {EMBL-User},
ddc = {540},
cid = {I:(DE-H253)EMBL-User-20120814},
pnm = {6G3 - PETRA III (POF3-622) / BIOSTRUCT-X - Transnational
access and enhancement of integrated Biological Structure
determination at synchrotron X-ray radiation facilities
(283570) / LASERLAB-EUROPE - The Integrated Initiative of
European Laser Research Infrastructures III (284464)},
pid = {G:(DE-HGF)POF3-6G3 / G:(EU-Grant)283570 /
G:(EU-Grant)284464},
experiment = {EXP:(DE-H253)P-P14-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28493496},
UT = {WOS:000404550000003},
doi = {10.1002/chem.201701591},
url = {https://bib-pubdb1.desy.de/record/397870},
}