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@ARTICLE{Meents:393805,
author = {Meents, Alke and Wiedorn, Max O. and Srajer, V. and
Henning, R. and Sarrou, Iosifina and Bergtholdt, Julian and
Barthelmess, Miriam and Reinke, P. Y. A. and Dierksmeyer,
Dennis and Tolstikova, A. and Schaible, S. and
Messerschmidt, M. and Ogata, C. M. and Kissick, D. J. and
Taft, M. H. and Manstein, D. J. and Lieske, Julia and
Oberthür, Dominik and Fischetti, R. F. and Chapman, Henry
N.},
title = {{P}ink-beam serial crystallography},
journal = {Nature Communications},
volume = {8},
number = {1},
issn = {2041-1723},
address = {London},
publisher = {Nature Publishing Group},
reportid = {PUBDB-2017-11676},
pages = {1281},
year = {2017},
abstract = {Serial X-ray crystallography allows macromolecular
structure determination at both X-ray free electron lasers
(XFELs) and, more recently, synchrotron sources. The time
resolution for serial synchrotron crystallography
experiments has been limited to millisecond timescales with
monochromatic beams. The polychromatic, “pink”, beam
provides a more than two orders of magnitude increased
photon flux and hence allows accessing much shorter
timescales in diffraction experiments at synchrotron
sources. Here we report the structure determination of two
different protein samples by merging pink-beam diffraction
patterns from many crystals, each collected with a single
100 ps X-ray pulse exposure per crystal using a setup
optimized for very low scattering background. In contrast to
experiments with monochromatic radiation, data from only 50
crystals were required to obtain complete datasets. The high
quality of the diffraction data highlights the potential of
this method for studying irreversible reactions at
sub-microsecond timescales using high-brightness X-ray
facilities.},
cin = {CFEL-I / FS-PS},
ddc = {500},
cid = {I:(DE-H253)CFEL-I-20161114 / I:(DE-H253)FS-PS-20131107},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
X-probe - Advanced XFEL and Synchrotron based Probes of
Protein Structure and Dynamics (637295) / BMBF-05K14CHA -
05K14CHA - SyncFELmed: Teilprojekt 2 (BMBF-05K14CHA) /
VH-VI-419 - Dynamic Pathways in Multidimensional Landscapes
(VH-VI-419) / VH-VI-403 - In-Situ Nano-Imaging of Biological
and Chemical Processes $(2015_IFV-VH-VI-403)$ / EUCALL -
European Cluster of Advanced Laser Light Sources (654220)},
pid = {G:(DE-HGF)POF3-6215 / G:(EU-Grant)637295 /
G:(DE-H253)BMBF-05K14CHA / G:(DE-HGF)VH-VI-419 /
$G:(DE-HGF)2015_IFV-VH-VI-403$ / G:(EU-Grant)654220},
experiment = {EXP:(DE-H253)CFEL-Exp-20150101 /
EXP:(DE-MLZ)External-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29097720},
UT = {WOS:000414376600002},
doi = {10.1038/s41467-017-01417-3},
url = {https://bib-pubdb1.desy.de/record/393805},
}