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@ARTICLE{Mayans:390618,
author = {Mayans, O. and van der Ven, P. F. and Wilm, M. and Mues, A.
and Young, P. and Fürst, D. O. and Wilmanns, M. and Gautel,
M.},
title = {{S}tructural basis for activation of the titin kinase
domain during myofibrillogenesis.},
journal = {Nature},
volume = {395},
number = {6705},
issn = {0028-0836},
address = {London [u.a.]},
publisher = {Nature Publ. Group},
reportid = {PUBDB-2017-09675},
pages = {863 - 869},
year = {1998},
note = {F-Bereich; EMBL},
abstract = {The giant muscle protein titin (connectin) is essential in
the temporal and spatial control of the assembly of the
highly ordered sarcomeres (contractile units) of striated
muscle. Here we present the crystal structure of titin's
only catalytic domain, an autoregulated serine kinase (titin
kinase). The structure shows how the active site is
inhibited by a tyrosine of the kinase domain. We describe a
dual mechanism of activation of titin kinase that consists
of phosphorylation of this tyrosine and binding of
calcium/calmodulin to the regulatory tail. The serine kinase
domain of titin is the first known non-arginine-aspartate
kinase to be activated by phosphorylation. The
phosphorylated tyrosine is not located in the activation
segment, as in other kinases, but in the P + 1 loop,
indicating that this tyrosine is a binding partner of the
titin kinase substrate. Titin kinase phosphorylates the
muscle protein telethonin in early differentiating myocytes,
indicating that this kinase may act in myofibrillogenesis.},
keywords = {Connectin (NLM Chemicals) / Muscle Proteins (NLM Chemicals)
/ Protein Kinase Inhibitors (NLM Chemicals) / Recombinant
Proteins (NLM Chemicals) / TCAP protein, human (NLM
Chemicals) / TTN protein, human (NLM Chemicals) / Tyrosine
(NLM Chemicals) / Protein Kinases (NLM Chemicals)},
cin = {DESY(-2012)},
ddc = {070},
cid = {$I:(DE-H253)DESY_-2012_-20170516$},
pnm = {899 - ohne Topic (POF3-899)},
pid = {G:(DE-HGF)POF3-899},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:9804419},
UT = {WOS:00},
doi = {10.1038/27603},
url = {https://bib-pubdb1.desy.de/record/390618},
}