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024 7 _ |a 10.1107/S1600577516018452
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100 1 _ |a Crosas, Eva
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245 _ _ |a Uridine as a new scavenger for synchrotron-based structural biology techniques
260 _ _ |a Chester
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520 _ _ |a Macromolecular crystallography (MX) and small-angle X-ray scattering (SAXS) studies on proteins at synchrotron light sources are commonly limited by the structural damage produced by the intense X-ray beam. Several effects, such as aggregation in protein solutions and global and site-specific damage in crystals, reduce the data quality or even introduce artefacts that can result in a biologically misguiding structure. One strategy to reduce these negative effects is the inclusion of an additive in the buffer solution to act as a free radical scavenger. Here the properties of uridine as a scavenger for both SAXS and MX experiments on lysozyme at room temperature are examined. In MX experiments, upon addition of uridine at 1 M, the critical dose D1/2 is increased by a factor of ~1.7, a value similar to that obtained in the presence of the most commonly used scavengers such as ascorbate and sodium nitrate. Other figures of merit to assess radiation damage show a similar trend. In SAXS experiments, the scavenging effect of 40 mM uridine is similar to that of 5% v/v glycerol, and greater than 2 mM DTT and 1 mM ascorbic acid. In all cases, the protective effect of uridine is proportional to its concentration.
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700 1 _ |a Castellvi, Albert
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700 1 _ |a Crespo, Isidro
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700 1 _ |a Fulla, Daniel
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700 1 _ |a Gil-Ortiz, Fernando
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700 1 _ |a Fuertes, Gustavo
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700 1 _ |a Kamma-Lorger, Christina
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700 1 _ |a Malfois, Marc
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700 1 _ |a Aranda, Miguel A. G.
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700 1 _ |a Juanhuix Gibert, Jordi
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773 _ _ |a 10.1107/S1600577516018452
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|t Journal of synchrotron radiation
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