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@ARTICLE{Crosas:329824,
      author       = {Crosas, Eva and Castellvi, Albert and Crespo, Isidro and
                      Fulla, Daniel and Gil-Ortiz, Fernando and Fuertes, Gustavo
                      and Kamma-Lorger, Christina and Malfois, Marc and Aranda,
                      Miguel A. G. and Juanhuix Gibert, Jordi},
      title        = {{U}ridine as a new scavenger for synchrotron-based
                      structural biology techniques},
      journal      = {Journal of synchrotron radiation},
      volume       = {24},
      number       = {1},
      issn         = {1600-5775},
      address      = {Chester},
      publisher    = {IUCr},
      reportid     = {PUBDB-2017-06374},
      pages        = {53 - 62},
      year         = {2017},
      abstract     = {Macromolecular crystallography (MX) and small-angle X-ray
                      scattering (SAXS) studies on proteins at synchrotron light
                      sources are commonly limited by the structural damage
                      produced by the intense X-ray beam. Several effects, such as
                      aggregation in protein solutions and global and
                      site-specific damage in crystals, reduce the data quality or
                      even introduce artefacts that can result in a biologically
                      misguiding structure. One strategy to reduce these negative
                      effects is the inclusion of an additive in the buffer
                      solution to act as a free radical scavenger. Here the
                      properties of uridine as a scavenger for both SAXS and MX
                      experiments on lysozyme at room temperature are examined. In
                      MX experiments, upon addition of uridine at 1 M, the
                      critical dose D1/2 is increased by a factor of ~1.7, a value
                      similar to that obtained in the presence of the most
                      commonly used scavengers such as ascorbate and sodium
                      nitrate. Other figures of merit to assess radiation damage
                      show a similar trend. In SAXS experiments, the scavenging
                      effect of 40 mM uridine is similar to that of $5\%$ v/v
                      glycerol, and greater than 2 mM DTT and 1 mM ascorbic acid.
                      In all cases, the protective effect of uridine is
                      proportional to its concentration.},
      cin          = {DOOR / EMBL-User},
      ddc          = {540},
      cid          = {I:(DE-H253)HAS-User-20120731 /
                      I:(DE-H253)EMBL-User-20120814},
      pnm          = {6G3 - PETRA III (POF3-622)},
      pid          = {G:(DE-HGF)POF3-6G3},
      experiment   = {EXP:(DE-H253)P-P12-20150101},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000391724900006},
      pubmed       = {pmid:28009546},
      doi          = {10.1107/S1600577516018452},
      url          = {https://bib-pubdb1.desy.de/record/329824},
}