TY  - JOUR
AU  - Crosas, Eva
AU  - Castellvi, Albert
AU  - Crespo, Isidro
AU  - Fulla, Daniel
AU  - Gil-Ortiz, Fernando
AU  - Fuertes, Gustavo
AU  - Kamma-Lorger, Christina
AU  - Malfois, Marc
AU  - Aranda, Miguel A. G.
AU  - Juanhuix Gibert, Jordi
TI  - Uridine as a new scavenger for synchrotron-based structural biology techniques
JO  - Journal of synchrotron radiation
VL  - 24
IS  - 1
SN  - 1600-5775
CY  - Chester
PB  - IUCr
M1  - PUBDB-2017-06374
SP  - 53 - 62
PY  - 2017
AB  - Macromolecular crystallography (MX) and small-angle X-ray scattering (SAXS) studies on proteins at synchrotron light sources are commonly limited by the structural damage produced by the intense X-ray beam. Several effects, such as aggregation in protein solutions and global and site-specific damage in crystals, reduce the data quality or even introduce artefacts that can result in a biologically misguiding structure. One strategy to reduce these negative effects is the inclusion of an additive in the buffer solution to act as a free radical scavenger. Here the properties of uridine as a scavenger for both SAXS and MX experiments on lysozyme at room temperature are examined. In MX experiments, upon addition of uridine at 1 M, the critical dose D1/2 is increased by a factor of  1.7, a value similar to that obtained in the presence of the most commonly used scavengers such as ascorbate and sodium nitrate. Other figures of merit to assess radiation damage show a similar trend. In SAXS experiments, the scavenging effect of 40 mM uridine is similar to that of 5
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000391724900006
C6  - pmid:28009546
DO  - DOI:10.1107/S1600577516018452
UR  - https://bib-pubdb1.desy.de/record/329824
ER  -