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000329824 245__ $$aUridine as a new scavenger for synchrotron-based structural biology techniques
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000329824 520__ $$aMacromolecular crystallography (MX) and small-angle X-ray scattering (SAXS) studies on proteins at synchrotron light sources are commonly limited by the structural damage produced by the intense X-ray beam. Several effects, such as aggregation in protein solutions and global and site-specific damage in crystals, reduce the data quality or even introduce artefacts that can result in a biologically misguiding structure. One strategy to reduce these negative effects is the inclusion of an additive in the buffer solution to act as a free radical scavenger. Here the properties of uridine as a scavenger for both SAXS and MX experiments on lysozyme at room temperature are examined. In MX experiments, upon addition of uridine at 1 M, the critical dose D1/2 is increased by a factor of ~1.7, a value similar to that obtained in the presence of the most commonly used scavengers such as ascorbate and sodium nitrate. Other figures of merit to assess radiation damage show a similar trend. In SAXS experiments, the scavenging effect of 40 mM uridine is similar to that of 5% v/v glycerol, and greater than 2 mM DTT and 1 mM ascorbic acid. In all cases, the protective effect of uridine is proportional to its concentration.
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000329824 7001_ $$0P:(DE-H253)PIP1023246$$aCastellvi, Albert$$b1
000329824 7001_ $$aCrespo, Isidro$$b2
000329824 7001_ $$aFulla, Daniel$$b3
000329824 7001_ $$aGil-Ortiz, Fernando$$b4
000329824 7001_ $$aFuertes, Gustavo$$b5
000329824 7001_ $$0P:(DE-H253)PIP1018504$$aKamma-Lorger, Christina$$b6
000329824 7001_ $$aMalfois, Marc$$b7
000329824 7001_ $$aAranda, Miguel A. G.$$b8
000329824 7001_ $$0P:(DE-H253)PIP1023239$$aJuanhuix Gibert, Jordi$$b9$$eCorresponding author
000329824 773__ $$0PERI:(DE-600)2021413-3$$a10.1107/S1600577516018452$$gVol. 24, no. 1, p. 53 - 62$$n1$$p53 - 62$$tJournal of synchrotron radiation$$v24$$x1600-5775$$y2017
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