%0 Journal Article
%A Crosas, Eva
%A Castellvi, Albert
%A Crespo, Isidro
%A Fulla, Daniel
%A Gil-Ortiz, Fernando
%A Fuertes, Gustavo
%A Kamma-Lorger, Christina
%A Malfois, Marc
%A Aranda, Miguel A. G.
%A Juanhuix Gibert, Jordi
%T Uridine as a new scavenger for synchrotron-based structural biology techniques
%J Journal of synchrotron radiation
%V 24
%N 1
%@ 1600-5775
%C Chester
%I IUCr
%M PUBDB-2017-06374
%P 53 - 62
%D 2017
%X Macromolecular crystallography (MX) and small-angle X-ray scattering (SAXS) studies on proteins at synchrotron light sources are commonly limited by the structural damage produced by the intense X-ray beam. Several effects, such as aggregation in protein solutions and global and site-specific damage in crystals, reduce the data quality or even introduce artefacts that can result in a biologically misguiding structure. One strategy to reduce these negative effects is the inclusion of an additive in the buffer solution to act as a free radical scavenger. Here the properties of uridine as a scavenger for both SAXS and MX experiments on lysozyme at room temperature are examined. In MX experiments, upon addition of uridine at 1 M, the critical dose D1/2 is increased by a factor of  1.7, a value similar to that obtained in the presence of the most commonly used scavengers such as ascorbate and sodium nitrate. Other figures of merit to assess radiation damage show a similar trend. In SAXS experiments, the scavenging effect of 40 mM uridine is similar to that of 5
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000391724900006
%$ pmid:28009546
%R 10.1107/S1600577516018452
%U https://bib-pubdb1.desy.de/record/329824