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@ARTICLE{Fercher:317093,
      author       = {Fercher, Christian and Probst, Ines and Kohler, Verena and
                      Goessweiner-Mohr, Nikolaus and Arends, Karsten and Grohmann,
                      Elisabeth and Zangger, Klaus and Meyer, N. Helge and Keller,
                      Walter},
      title        = {{V}ir{B}8-like protein {T}ra{H} is crucial for {DNA}
                      transfer in {E}nterococcus faecalis},
      journal      = {Scientific reports},
      volume       = {6},
      issn         = {2045-2322},
      address      = {London},
      publisher    = {Nature Publishing Group},
      reportid     = {PUBDB-2017-00434},
      pages        = {24643},
      year         = {2016},
      abstract     = {Untreatable bacterial infections caused by a perpetual
                      increase of antibiotic resistant strains represent a serious
                      threat to human healthcare in the 21st century. Conjugative
                      DNA transfer is the most important mechanism for antibiotic
                      resistance and virulence gene dissemination among bacteria
                      and is mediated by a protein complex, known as type IV
                      secretion system (T4SS). The core of the T4SS is a
                      multiprotein complex that spans the bacterial envelope as a
                      channel for macromolecular secretion. We report the NMR
                      structure and functional characterization of the transfer
                      protein TraH encoded by the conjugative Gram-positive
                      broad-host range plasmid pIP501. The structure exhibits a
                      striking similarity to VirB8 proteins of Gram-negative
                      secretion systems where they play an essential role in the
                      scaffold of the secretion machinery. Considering TraM as the
                      first VirB8-like protein discovered in pIP501, TraH
                      represents the second protein affiliated with this family in
                      the respective transfer operon. A markerless traH deletion
                      in pIP501 resulted in a total loss of transfer in
                      Enterococcus faecalis as compared with the pIP501 wild type
                      (wt) plasmid, demonstrating that TraH is essential for
                      pIP501 mediated conjugation. Moreover, oligomerization state
                      and topology of TraH in the native membrane were determined
                      providing insights in molecular organization of a
                      Gram-positive T4SS.},
      cin          = {DOOR ; HAS-User / CSSB-UKE / CSSB-UKE-TM},
      ddc          = {000},
      cid          = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)CSSB-UKE-20141216
                      / I:(DE-H253)CSSB-UKE-TM-20210520},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      experiment   = {EXP:(DE-H253)D-D1.2-20150101},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000374540100001},
      pubmed       = {pmid:27103580},
      doi          = {10.1038/srep24643},
      url          = {https://bib-pubdb1.desy.de/record/317093},
}