Home > Publications database > Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator > print

001     317072
005     20250730111819.0
024 7 _ |a 10.1093/nar/gkw191
|2 doi
024 7 _ |a 0261-3166
|2 ISSN
024 7 _ |a 0305-1048
|2 ISSN
024 7 _ |a 1362-4962
|2 ISSN
024 7 _ |a 1746-8272
|2 ISSN
024 7 _ |a 10.3204/PUBDB-2017-00413
|2 datacite_doi
024 7 _ |a WOS:000379754600043
|2 WOS
024 7 _ |a pmid:27016739
|2 pmid
024 7 _ |a altmetric:6349983
|2 altmetric
024 7 _ |a openalex:W2322495435
|2 openalex
037 _ _ |a PUBDB-2017-00413
041 _ _ |a English
082 _ _ |a 540
100 1 _ |a Rajasekar, Karthik V.
|0 P:(DE-HGF)0
|b 0
245 _ _ |a Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator
260 _ _ |a Oxford
|c 2016
|b Oxford Univ. Press
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1484666748_9488
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a The IncP (Incompatibility group P) plasmids are important carriers in the spread of antibiotic resistance across Gram-negative bacteria. Gene expression in the IncP-1 plasmids is stringently controlled by a network of four global repressors, KorA, KorB, TrbA and KorC interacting cooperatively. Intriguingly, KorA and KorB can act as co-repressors at varying distances between their operators, even when they are moved to be on opposite sides of the DNA. KorA is a homodimer with the 101-amino acid subunits, folding into an N-terminal DNA-binding domain and a C-terminal dimerization domain. In this study, we have determined the structures of the free KorA repressor and two complexes each bound to a 20-bp palindromic DNA duplex containing its consensus operator sequence. Using a combination of X-ray crystallography, nuclear magnetic resonance spectroscopy, SAXS and molecular dynamics calculations, we show that the linker between the two domains is very flexible and the protein remains highly mobile in the presence of DNA. This flexibility allows the DNA-binding domains of the dimer to straddle the operator DNA on binding and is likely to be important in cooperative binding to KorB. Unexpectedly, the C-terminal domain of KorA is structurally similar to the dimerization domain of the tumour suppressor p53.
536 _ _ |a 899 - ohne Topic (POF3-899)
|0 G:(DE-HGF)POF3-899
|c POF3-899
|f POF III
|x 0
588 _ _ |a Dataset connected to CrossRef
693 _ _ |a DORIS III
|e Facility (machine) DORIS III
|1 EXP:(DE-H253)DORISIII-20150101
|0 EXP:(DE-H253)DORISIII(machine)-20150101
|5 EXP:(DE-H253)DORISIII(machine)-20150101
|x 0
700 1 _ |a Lovering, Andrew L.
|0 P:(DE-HGF)0
|b 1
700 1 _ |a Dancea, Felician
|0 P:(DE-HGF)0
|b 2
700 1 _ |a Scott, David J.
|0 P:(DE-HGF)0
|b 3
700 1 _ |a Harris, Sarah A.
|0 P:(DE-HGF)0
|b 4
700 1 _ |a Bingle, Lewis E. H.
|0 P:(DE-HGF)0
|b 5
700 1 _ |a Roessle, Manfred
|0 P:(DE-H253)PIP1003579
|b 6
700 1 _ |a Thomas, Christopher M.
|0 P:(DE-HGF)0
|b 7
700 1 _ |a Hyde, Eva I.
|0 P:(DE-HGF)0
|b 8
|e Corresponding author
700 1 _ |a White, Scott A.
|0 P:(DE-HGF)0
|b 9
|e Corresponding author
773 _ _ |a 10.1093/nar/gkw191
|g Vol. 44, no. 10, p. 4947 - 4956
|0 PERI:(DE-600)2205588-5
|n 10
|p 4947 - 4956
|t Nucleic acids symposium series
|v 44
|y 2016
|x 1362-4962
856 4 _ |y OpenAccess
|u https://bib-pubdb1.desy.de/record/317072/files/gkw191.pdf
856 4 _ |y OpenAccess
|x icon
|u https://bib-pubdb1.desy.de/record/317072/files/gkw191.gif?subformat=icon
856 4 _ |y OpenAccess
|x icon-1440
|u https://bib-pubdb1.desy.de/record/317072/files/gkw191.jpg?subformat=icon-1440
856 4 _ |y OpenAccess
|x icon-180
|u https://bib-pubdb1.desy.de/record/317072/files/gkw191.jpg?subformat=icon-180
856 4 _ |y OpenAccess
|x icon-640
|u https://bib-pubdb1.desy.de/record/317072/files/gkw191.jpg?subformat=icon-640
909 C O |o oai:bib-pubdb1.desy.de:317072
|p openaire
|p open_access
|p VDB
|p driver
|p dnbdelivery
910 1 _ |a Europäisches Laboratorium für Molekularbiologie
|0 I:(DE-588b)235011-7
|k EMBL
|b 6
|6 P:(DE-H253)PIP1003579
913 1 _ |a DE-HGF
|b Programmungebundene Forschung
|l ohne Programm
|1 G:(DE-HGF)POF3-890
|0 G:(DE-HGF)POF3-899
|2 G:(DE-HGF)POF3-800
|v ohne Topic
|x 0
|4 G:(DE-HGF)POF
|3 G:(DE-HGF)POF3
914 1 _ |y 2016
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
915 _ _ |a Creative Commons Attribution CC BY 4.0
|0 LIC:(DE-HGF)CCBY4
|2 HGFVOC
915 _ _ |a OpenAccess
|0 StatID:(DE-HGF)0510
|2 StatID
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b NUCLEIC ACIDS RES : 2015
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0501
|2 StatID
|b DOAJ Seal
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0500
|2 StatID
|b DOAJ
915 _ _ |a WoS
|0 StatID:(DE-HGF)0110
|2 StatID
|b Science Citation Index
915 _ _ |a WoS
|0 StatID:(DE-HGF)0111
|2 StatID
|b Science Citation Index Expanded
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
915 _ _ |a Allianz-Lizenz / DFG
|0 StatID:(DE-HGF)0400
|2 StatID
915 _ _ |a IF >= 5
|0 StatID:(DE-HGF)9905
|2 StatID
|b NUCLEIC ACIDS RES : 2015
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0310
|2 StatID
|b NCBI Molecular Biology Database
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
915 _ _ |a Nationallizenz
|0 StatID:(DE-HGF)0420
|2 StatID
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Thomson Reuters Master Journal List
920 1 _ |0 I:(DE-H253)EMBL-20120731
|k EMBL
|l EMBL
|x 0
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a UNRESTRICTED
980 _ _ |a I:(DE-H253)EMBL-20120731
980 1 _ |a FullTexts

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21