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@INPROCEEDINGS{Mishra:316551,
      author       = {Mishra, Seema and Kuepper, Hendrik and Alfeld, Matthias and
                      Sobotka, Roman and Mattusch, Jürgen},
      title        = {{T}ransformation and {S}ubcellular {D}istribution of
                      {A}rsenic and {M}echanism of its {S}ub-lethal {T}oxicity in
                      {P}lants},
      school       = {CSIR-National Botanical Research Institute, Lucknow, India},
      reportid     = {PUBDB-2016-06710},
      year         = {2016},
      abstract     = {Speciation of arsenic (As) in rice, the most As affected
                      crop, and Ceratophyllum demersum, a good laboratorymodel for
                      shoot, through chromatography and localization in the later
                      through synchrotron based techniqueswas performed to explore
                      the mechanism of As toxicity. In rice plant, exposed to
                      inorganic arsenate (AsV) andmethylated As, most of the AsV
                      and methylarsonate (MAV) were efficiently reduced to
                      arsenite (AsIII) and MAIIIrespectively, but dimethylarsinate
                      (DMAV) did not transformed. A large proportion of AsIII and
                      MAIII were complexedwith thiols showing up to 20 and 16 As
                      species respectively in the roots. Many of them were
                      identifiedas new As-thiol species. Despite high complexation
                      in root, more MA was translocated to shoot, with
                      shoot/roottransfer factor being in order DMA>MA>inorganic As
                      in rice.C. demersum, also displayed up to $60\%$ accumulated
                      As in the form of thiol complexed-AsIII. Most of which wasin
                      epidermis of mature leaves as revealed by tissue resolved
                      X-ray absorption near-edge spectroscopy (μ-XANES) of intact
                      hydrated leaves. At sublethal concentration, As
                      predominantly accumulated in the nucleus ofthe epidermal
                      cells, as revealed by μ-X-ray fluorescence (μ-XRF),
                      indicating replacement of P by As in DNA molecules,providing
                      in vivo evidence for the proposed toxicity mechanism of AsV.
                      While at lethal concentration,vacuole was the main storage
                      site of As, yet a significant increase of unbound AsIII in
                      mesophylls of young matureleaves occurred. This small amount
                      of As reaching chloroplasts already caused a strong and
                      specific inhibitionof tetrapyrrole biosynthesis and severe
                      growth retardation.Taken together these results establish
                      the mechanism of As toxicity and reported yield loss in
                      paddy rice growingin As contaminated areas. Further,
                      significant translocation of MAIII (more cytotoxic than
                      AsIII) to rice shootscould also be an important factor
                      inducing straighthead (spikelet sterility disorder) in
                      rice.},
      month         = {Jun},
      date          = {2016-06-26},
      organization  = {Plant Biology Europe EPSO/FESPB 2016
                       Congress, Prague (Czech Republic), 26
                       Jun 2016 - 30 Jun 2016},
      subtyp        = {After Call},
      cin          = {DOOR},
      cid          = {I:(DE-H253)HAS-User-20120731},
      pnm          = {6G3 - PETRA III (POF3-622) / FS-Proposal: I-20110682
                      (I-20110682) / FS-Proposal: I-20120397 (I-20120397) /
                      FS-Proposal: I-20130425 (I-20130425)},
      pid          = {G:(DE-HGF)POF3-6G3 / G:(DE-H253)I-20110682 /
                      G:(DE-H253)I-20120397 / G:(DE-H253)I-20130425},
      experiment   = {EXP:(DE-H253)D-L-20150101 / EXP:(DE-H253)P-P06-20150101},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://bib-pubdb1.desy.de/record/316551},
}