000316551 001__ 316551
000316551 005__ 20210406113357.0
000316551 037__ $$aPUBDB-2016-06710
000316551 041__ $$aEnglish
000316551 1001_ $$0P:(DE-H253)PIP1015493$$aMishra, Seema$$b0$$eCorresponding author
000316551 1112_ $$aPlant Biology Europe EPSO/FESPB 2016 Congress$$cPrague$$d2016-06-26 - 2016-06-30$$wCzech Republic
000316551 245__ $$aTransformation and Subcellular Distribution of Arsenic and Mechanism of its Sub-lethal Toxicity in Plants
000316551 260__ $$c2016
000316551 3367_ $$033$$2EndNote$$aConference Paper
000316551 3367_ $$2DataCite$$aOther
000316551 3367_ $$2BibTeX$$aINPROCEEDINGS
000316551 3367_ $$2DRIVER$$aconferenceObject
000316551 3367_ $$2ORCID$$aLECTURE_SPEECH
000316551 3367_ $$0PUB:(DE-HGF)6$$2PUB:(DE-HGF)$$aConference Presentation$$bconf$$mconf$$s1484218552_14942$$xAfter Call
000316551 502__ $$cCSIR-National Botanical Research Institute, Lucknow, India
000316551 520__ $$aSpeciation of arsenic (As) in rice, the most As affected crop, and Ceratophyllum demersum, a good laboratorymodel for shoot, through chromatography and localization in the later through synchrotron based techniqueswas performed to explore the mechanism of As toxicity. In rice plant, exposed to inorganic arsenate (AsV) andmethylated As, most of the AsV and methylarsonate (MAV) were efficiently reduced to arsenite (AsIII) and MAIIIrespectively, but dimethylarsinate (DMAV) did not transformed. A large proportion of AsIII and MAIII were complexedwith thiols showing up to 20 and 16 As species respectively in the roots. Many of them were identifiedas new As-thiol species. Despite high complexation in root, more MA was translocated to shoot, with shoot/roottransfer factor being in order DMA>MA>inorganic As in rice.C. demersum, also displayed up to 60% accumulated As in the form of thiol complexed-AsIII. Most of which wasin epidermis of mature leaves as revealed by tissue resolved X-ray absorption near-edge spectroscopy (μ-XANES) of intact hydrated leaves. At sublethal concentration, As predominantly accumulated in the nucleus ofthe epidermal cells, as revealed by μ-X-ray fluorescence (μ-XRF), indicating replacement of P by As in DNA molecules,providing in vivo evidence for the proposed toxicity mechanism of AsV. While at lethal concentration,vacuole was the main storage site of As, yet a significant increase of unbound AsIII in mesophylls of young matureleaves occurred. This small amount of As reaching chloroplasts already caused a strong and specific inhibitionof tetrapyrrole biosynthesis and severe growth retardation.Taken together these results establish the mechanism of As toxicity and reported yield loss in paddy rice growingin As contaminated areas. Further, significant translocation of MAIII (more cytotoxic than AsIII) to rice shootscould also be an important factor inducing straighthead (spikelet sterility disorder) in rice.
000316551 536__ $$0G:(DE-HGF)POF3-6G3$$a6G3 - PETRA III (POF3-622)$$cPOF3-622$$fPOF III$$x0
000316551 536__ $$0G:(DE-H253)I-20110682$$aFS-Proposal: I-20110682 (I-20110682)$$cI-20110682$$x1
000316551 536__ $$0G:(DE-H253)I-20120397$$aFS-Proposal: I-20120397 (I-20120397)$$cI-20120397$$x2
000316551 536__ $$0G:(DE-H253)I-20130425$$aFS-Proposal: I-20130425 (I-20130425)$$cI-20130425$$x3
000316551 693__ $$0EXP:(DE-H253)D-L-20150101$$1EXP:(DE-H253)DORISIII-20150101$$6EXP:(DE-H253)D-L-20150101$$aDORIS III$$fDORIS Beamline L$$x0
000316551 693__ $$0EXP:(DE-H253)P-P06-20150101$$1EXP:(DE-H253)PETRAIII-20150101$$6EXP:(DE-H253)P-P06-20150101$$aPETRA III$$fPETRA Beamline P06$$x1
000316551 7001_ $$0P:(DE-H253)PIP1013392$$aKuepper, Hendrik$$b1
000316551 7001_ $$0P:(DE-H253)PIP1007955$$aAlfeld, Matthias$$b2
000316551 7001_ $$0P:(DE-HGF)0$$aSobotka, Roman$$b3
000316551 7001_ $$0P:(DE-HGF)0$$aMattusch, Jürgen$$b4
000316551 8564_ $$uhttp://www.europlantbiology2016.org/scientific-programme/
000316551 909CO $$ooai:bib-pubdb1.desy.de:316551$$pVDB
000316551 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1015493$$aExternes Institut$$b0$$kExtern
000316551 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1013392$$aExternes Institut$$b1$$kExtern
000316551 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1007955$$aExternes Institut$$b2$$kExtern
000316551 9131_ $$0G:(DE-HGF)POF3-622$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6G3$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vFacility topic: Research on Matter with Brilliant Light Sources$$x0
000316551 9141_ $$y2016
000316551 9201_ $$0I:(DE-H253)HAS-User-20120731$$kDOOR$$lDOOR-User$$x0
000316551 980__ $$aconf
000316551 980__ $$aVDB
000316551 980__ $$aI:(DE-H253)HAS-User-20120731
000316551 980__ $$aUNRESTRICTED