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000315234 1001_ $$0P:(DE-H253)PIP1008276$$aSchlathoelter, Thomas$$b0$$eCorresponding author
000315234 245__ $$aMultiple Ionization of Free Ubiquitin Molecular Ions in Extreme Ultraviolet Free-Electron Laser Pulses
000315234 260__ $$aWeinheim$$bWiley-VCH9102$$c2016
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000315234 500__ $$aInternational edition: 10.1002/anie.201605335
000315234 520__ $$aThe fragmentation of free tenfold protonated ubiquitin in intense 70 femtosecond pulses of 90 eV photons from the FLASH facility was investigated. Mass spectrometric investigation of the fragment cations produced after removal of many electrons revealed fragmentation predominantly into immonium ions and related ions, with yields increasing linearly with intensity. Ionization clearly triggers a localized molecular response that occurs before the excitation energy equilibrates. Consistent with this interpretation, the effect is almost unaffected by the charge state, as fragmentation of sixfold deprotonated ubiquitin leads to a very similar fragmentation pattern. Ubiquitin responds to EUV multiphoton ionization as an ensemble of small peptides.
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000315234 7001_ $$0P:(DE-H253)PIP1017117$$aReitsma, Geert$$b1
000315234 7001_ $$0P:(DE-H253)PIP1029403$$aEgorov, Dmitrii$$b2
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000315234 7001_ $$0P:(DE-H253)PIP1008383$$aSchröter, Claus Dieter$$b9
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000315234 773__ $$0PERI:(DE-600)2011836-3$$a10.1002/ange.201605335$$gVol. 128, no. 36, p. 10899 - 10903$$n36$$p10899 - 10903$$tAngewandte Chemie / International edition$$v128$$x0044-8249$$y2016
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