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000315131 0247_ $$2ISSN$$a2053-230X
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000315131 0247_ $$2datacite_doi$$a10.3204/PUBDB-2016-05653
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000315131 041__ $$aEnglish
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000315131 1001_ $$0P:(DE-H253)PIP1027725$$aGrinkevich, Pavel$$b0
000315131 245__ $$apHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I
000315131 260__ $$bIUCr$$c2016
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000315131 520__ $$aThe HsdR subunit of the type I restriction-modification system EcoR124I is responsible for the translocation as well as the restriction activity of the whole complex consisting of the HsdR, HsdM and HsdS subunits, and while crystal structures are available for the wild type and several mutants, the C-terminal domain comprising approximately 150 residues was not resolved in any of these structures. Here, three fusion constructs with the GFP variant pHluorin developed to overexpress, purify and crystallize the C-terminal domain of HsdR are reported. The shortest of the three encompassed HsdR residues 887-1038 and yielded crystals that belonged to the orthorhombic space group C222$_1$, with unit-cell parameters a = 83.42, b = 176.58, c = 126.03 Å, $\alpha$ = $\beta$ = $\gamma$ = 90.00° and two molecules in the asymmetric unit (V$_M$ = 2.55 Å$^3$ Da$^{-1}$, solvent content 50.47%). X-ray diffraction data were collected to a resolution of 2.45 Å.
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000315131 536__ $$0G:(EU-Grant)283570$$aBIOSTRUCT-X - Transnational access and enhancement of integrated Biological Structure determination at synchrotron X-ray radiation facilities (283570)$$c283570$$fFP7-INFRASTRUCTURES-2011-1$$x1
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000315131 693__ $$0EXP:(DE-H253)P-P13-20150101$$1EXP:(DE-H253)PETRAIII-20150101$$6EXP:(DE-H253)P-P13-20150101$$aPETRA III$$fPETRA Beamline P13$$x0
000315131 7001_ $$0P:(DE-H253)PIP1027726$$aIermak, Iuliia$$b1
000315131 7001_ $$0P:(DE-HGF)0$$aLuedtke, Nicholas A.$$b2
000315131 7001_ $$0P:(DE-HGF)0$$aMesters, Jeroen R.$$b3
000315131 7001_ $$0P:(DE-HGF)0$$aEttrich, Rüdiger$$b4$$eCorresponding author
000315131 7001_ $$0P:(DE-HGF)0$$aLudwig, Jost$$b5
000315131 773__ $$0PERI:(DE-600)2175956-X$$a10.1107/S2053230X16011626$$gVol. 72, no. 9, p. 672 - 676$$n9$$p672 - 676$$tActa crystallographica / F$$v72$$x2053-230X$$y2016
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000315131 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a Institute of Biochemistry, University of Lübeck$$b3
000315131 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a Center for Nanobiology and Structural Biology, Institute of Microbiology ASCR$$b4
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000315131 9141_ $$y2016
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