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@ARTICLE{Zhou:311643,
author = {Zhou, X. Edward and Gao, Xiang and Barty, Anton and Kang,
Yanyong and He, Yuanzheng and liu, wei and Ishchenko, Andrii
and White, Thomas and Yefanov, Oleksandr and Han, Gye Won
and Xu, Qingping and de Waal, Parker W. and Suino-Powell,
Kelly M. and Boutet, Sébastien and Williams, Garth J. and
Wang, Meitian and Li, Dianfan and Caffrey, Martin and
Chapman, Henry N. and Spence, John C. H. and Fromme, Petra
and Weierstall, Uwe and Stevens, Raymond C. and Cherezov,
Vadim and Melcher, Karsten and Xu, H. Eric},
title = {{X}-ray laser diffraction for structure determination of
the rhodopsin-arrestin complex},
journal = {Scientific data},
volume = {3},
issn = {2052-4463},
address = {London},
publisher = {Nature Publ. Group},
reportid = {PUBDB-2016-04951},
pages = {160021},
year = {2016},
note = {URL of special issue:
http://www.nature.com/sdata/collections/xfel-biodata},
abstract = {Serial femtosecond X-ray crystallography (SFX) using an
X-ray free electron laser (XFEL) is a recent advancement in
structural biology for solving crystal structures of
challenging membrane proteins, including G-protein coupled
receptors (GPCRs), which often only produce microcrystals.
An XFEL delivers highly intense X-ray pulses of femtosecond
duration short enough to enable the collection of single
diffraction images before significant radiation damage to
crystals sets in. Here we report the deposition of the XFEL
data and provide further details on crystallization, XFEL
data collection and analysis, structure determination, and
the validation of the structural model. The
rhodopsin-arrestin crystal structure solved with SFX
represents the first near-atomic resolution structure of a
GPCR-arrestin complex, provides structural insights into
understanding of arrestin-mediated GPCR signaling, and
demonstrates the great potential of this SFX-XFEL technology
for accelerating crystal structure determination of
challenging proteins and protein complexes.},
cin = {FS-CFEL-1 / CFEL-I},
ddc = {610},
cid = {I:(DE-H253)FS-CFEL-1-20120731 / I:(DE-H253)CFEL-I-20161114},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
CUI - Hamburger Zentrum für ultraschnelle Beobachtung
(194651731)},
pid = {G:(DE-HGF)POF3-6215 / G:(GEPRIS)194651731},
experiment = {EXP:(DE-MLZ)External-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000390214400001},
pubmed = {pmid:27070998},
doi = {10.1038/sdata.2016.21},
url = {https://bib-pubdb1.desy.de/record/311643},
}